Arsenic resistance strategy in Pantoea sp. IMH: Organization, function and evolution of ars genes.
Arsenic resistance strategy in Pantoea sp. IMH: Organization, function and evolution of ars genes.
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Pantoea sp. 的砷抗性策略。
DOI：
10.1038/srep39195
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发表时间：
2016-12-14
影响因子：
4.6
通讯作者：
Jing C
中科院分区：
文献类型：
--
作者：
Wang L;Zhuang X;Zhuang G;Jing C
泛菌属（Pantoea sp. IMH）是泛菌属（Pantoea）中唯一具有高抗砷能力的细菌，但其抗砷的分子机制尚不清楚。本文从基因组全序列分析入手，对IMH中ars基因的组织、功能和进化进行了研究。在IMH基因组中发现了两个低同源性的ars系统ars1（arsR1B1C1H1）和ars2（arsR2B2C2H2）以及两个arsC样基因。ars1和ars2都参与了抗As性，其中ars1在15 °C时是主要贡献者，ars2在30 °C时是主要贡献者。这两个ars系统的行为差异归因于它们的arsR启动子在不同温度下的不同活性。基于串联ArsRBC的序列分析表明，ars1和ars2簇可以通过水平基因转移（HGT）分别从瑞士弗朗哥杆菌LMG 23732和粘质沙雷氏菌（质粒R478）获得。然而，两个arsC样基因，可能产生的arsC的重复，不有助于作为电阻。我们的研究结果表明，Pantoea sp. IMH收购两个不同的抗砷遗传系统的HGT，允许殖民化的不断变化的生态系统，并突出了灵活的适应微生物抵抗As。
Pantoea sp. IMH is the only bacterium found in genus Pantoea with a high As resistance capacity, but its molecular mechanism is unknown. Herein, the organization, function, and evolution of ars genes in IMH are studied starting with analysis of the whole genome. Two ars systems - ars1 (arsR1B1C1H1) and ars2 (arsR2B2C2H2) - with low sequence homology and two arsC-like genes, were found in the IMH genome. Both ars1 and ars2 are involved in the As resistance, where ars1 is the major contributor at 15 °C and ars2 at 30 °C. The difference in the behavior of these two ars systems is attributed to the disparate activities of their arsR promoters at different temperatures. Sequence analysis based on concatenated ArsRBC indicates that ars1 and ars2 clusters may be acquired from Franconibacter helveticus LMG23732 and Serratia marcescens (plasmid R478), respectively, by horizontal gene transfer (HGT). Nevertheless, two arsC-like genes, probably arising from the duplication of arsC, do not contribute to the As resistance. Our results indicate that Pantoea sp. IMH acquired two different As resistance genetic systems by HGT, allowing the colonization of changing ecosystems, and highlighting the flexible adaptation of microorganisms to resist As.
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影响因子：
3.5
作者：
Paez-Espino, David;Tamames, Javier;Canovas, David
通讯作者：
Canovas, David
影响因子：
4.5
作者：
Arsène-Ploetze F;Koechler S;Marchal M;Coppée JY;Chandler M;Bonnefoy V;Brochier-Armanet C;Barakat M;Barbe V;Battaglia-Brunet F;Bruneel O;Bryan CG;Cleiss-Arnold J;Cruveiller S;Erhardt M;Heinrich-Salmeron A;Hommais F;Joulian C;Krin E;Lieutaud A;Lièvremont D;Michel C;Muller D;Ortet P;Proux C;Siguier P;Roche D;Rouy Z;Salvignol G;Slyemi D;Talla E;Weiss S;Weissenbach J;Médigue C;Bertin PN
通讯作者：
Bertin PN
影响因子：
5.1
作者：
David Paez-Espino, A.;Durante-Rodriguez, Gonzalo;de Lorenzo, Victor
通讯作者：
de Lorenzo, Victor
影响因子：
14.9
作者：
Lagesen K;Hallin P;Rødland EA;Staerfeldt HH;Rognes T;Ussery DW
通讯作者：
Ussery DW
影响因子：
4.8
作者：
Gladysheva, T;Liu, JY;Rosen, BP
通讯作者：
Rosen, BP