Structure, function, and evolution of the Thiomonas spp. genome.
Structure, function, and evolution of the Thiomonas spp. genome.
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DOI：
10.1371/journal.pgen.1000859
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发表时间：
2010-02-26
期刊：
影响因子：
4.5
通讯作者：
Bertin PN
中科院分区：
文献类型：
--
作者：
Arsène-Ploetze F;Koechler S;Marchal M;Coppée JY;Chandler M;Bonnefoy V;Brochier-Armanet C;Barakat M;Barbe V;Battaglia-Brunet F;Bruneel O;Bryan CG;Cleiss-Arnold J;Cruveiller S;Erhardt M;Heinrich-Salmeron A;Hommais F;Joulian C;Krin E;Lieutaud A;Lièvremont D;Michel C;Muller D;Ortet P;Proux C;Siguier P;Roche D;Rouy Z;Salvignol G;Slyemi D;Talla E;Weiss S;Weissenbach J;Médigue C;Bertin PN
硫单胞菌属的细菌普遍存在于极端环境中，例如富砷酸性矿井排水（AMD）。对其中一种菌株硫单胞菌属3As的基因组进行了测序、注释和检查，揭示了允许这种细菌在其高毒性环境中生存和生长的特定适应性。为了探讨硫单胞菌属的基因组多样性和遗传进化，比较基因组杂交（CGH）方法用于硫单胞菌属的八种不同菌株，包括相同物种的五种菌株。我们的研究结果表明，硫单胞菌基因组已经通过基因组岛的获得或丧失而进化，并且这种进化受到菌株生活的特定环境条件的影响。砷微生物代谢领域的最新进展表明，细菌在大量高污染环境中定植。硫单胞菌属属于伯克霍尔德菌目，在砷污染环境中普遍存在。其中之一，即硫单胞菌属3As的基因组，被破译，并与其他几个硫单胞菌菌株的基因组进行比较。我们发现，它们灵活的基因库进化成允许它们在特殊的环境中生存和生长。特别是，收购的菌株相同的物种不同的基因组岛赋予重金属抗性和代谢特性。我们的比较基因组分析表明，自然环境影响这些细菌的基因组进化。重要的是，这些结果突出了可能存在于分类组内部的基因组变异性，扩大了细菌物种的概念。
Bacteria of the Thiomonas genus are ubiquitous in extreme environments, such as arsenic-rich acid mine drainage (AMD). The genome of one of these strains, Thiomonas sp. 3As, was sequenced, annotated, and examined, revealing specific adaptations allowing this bacterium to survive and grow in its highly toxic environment. In order to explore genomic diversity as well as genetic evolution in Thiomonas spp., a comparative genomic hybridization (CGH) approach was used on eight different strains of the Thiomonas genus, including five strains of the same species. Our results suggest that the Thiomonas genome has evolved through the gain or loss of genomic islands and that this evolution is influenced by the specific environmental conditions in which the strains live. Recent advances in the field of arsenic microbial metabolism have revealed that bacteria colonize a large panel of highly contaminated environments. Belonging to the order of Burkholderiales, Thiomonas strains are ubiquitous in arsenic-contaminated environments. The genome of one of them, i.e. Thiomonas sp. 3As, was deciphered and compared to the genome of several other Thiomonas strains. We found that their flexible gene pool evolved to allow both the surviving and growth in their peculiar environment. In particular, the acquisition by strains of the same species of different genomic islands conferred heavy metal resistance and metabolic idiosyncrasies. Our comparative genomic analyses suggest that the natural environment influences the genomic evolution of these bacteria. Importantly, these results highlight the genomic variability that may exist inside a taxonomic group, enlarging the concept of bacterial species.
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影响因子：
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作者：
Battaglia-Brunet, F;Dictor, MC;Baranger, P
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