A serological assay to detect human SARS-CoV-2 antibodies.
A serological assay to detect human SARS-CoV-2 antibodies.
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一种检测人SARS-CoV-2抗体的血清学方法
DOI：
10.1016/j.jtumed.2020.11.011
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发表时间：
2021-03
影响因子：
2.2
通讯作者：
Mahallawi WH
中科院分区：
文献类型：
--
作者：
Mahallawi WH
严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）是2019冠状病毒病（COVID-19）的病原体，可导致严重的呼吸道疾病。患有基础合并症的患者感染COVID-19的风险很高。因此，迫切需要血清学检测来诊断SARS-CoV-2的无症状携带者，以估计感染的患病率，并用于疾病预防和控制。本研究旨在建立一种酶联免疫吸附试验（ELISA）检测人血清中抗SARS-CoV-2抗体的方法。设计并建立了一种ELISA检测方法，用于检测41例定量逆转录聚合酶链反应（qRT-PCR）阳性住院COVID-19患者血清样本中的SARS-CoV-2刺突蛋白抗体。42名康复期患者的血清作为阳性对照，而117份大流行前的血清样本作为阴性对照。对不同SARS-CoV-2蛋白进行了比较，其中包括全长刺突（S）蛋白以及S1和S2亚基。全长S蛋白与患者血清中抗SARS-CoV-2 IgG抗体的反应性最强。此外，由于可以在非常低的浓度下检测到抗体，因此发现该测定法具有灵敏度。由于没有发现与其他SARS冠状病毒和流感等呼吸道病毒的交叉反应，因此目前的检测方法具有特异性。此外，由于该检测方法即使在非常低的浓度下也能够识别抗体，因此具有高度敏感性。因此，该检测方法有望作为人群水平的筛查方法，并可用于未来的血清流行病学研究。
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19), can lead to severe respiratory illness. Patients with underlying comorbidities have a high risk of contracting COVID-19. Therefore, serological assays are urgently needed to diagnose asymptomatic carriers of SARS-CoV-2, to estimate the prevalence of infection, and for disease prevention and control. This study aimed to develop an enzyme-linked immunosorbent assay (ELISA) for the detection of anti-SARS-CoV-2 antibodies in humans. An ELISA test was designed and established to detect antibodies against the SARS-CoV-2 spike protein in serum samples from 41 quantitative reverse transcription polymerase chain reaction (qRT-PCR) - positive hospitalised COVID-19 patients. Forty-two convalescent patients' sera served as positive controls, while 117 pre-pandemic serum samples were used as negative controls. A comparison between different SARS-CoV-2 proteins was performed, which included the full-length spike (S) protein and the S1 and S2 subunits. The full-length S protein showed the strongest reactivity for anti-SARS-CoV-2 IgG antibodies in patients' serum samples. Additionally, since antibodies could be detected at very low concentrations, the assay was found to be sensitive. The current assay was specific, since cross-reactions with other SARS coronaviruses and respiratory viruses such as influenza were not found. Additionally, it was highly sensitive, since the test was able to identify antibodies even at very low concentrations. Therefore, this assay has promise as a screening method at the population level and may be used for in future seroepidemiological studies.
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酶联免疫吸附测定法，用于对植物次生代谢产物的定量/定性分析。
影响因子：
3.3
作者：
Sakamoto S;Putalun W;Vimolmangkang S;Phoolcharoen W;Shoyama Y;Tanaka H;Morimoto S
通讯作者：
Morimoto S
影响因子：
82.9
作者：
Amanat F;Stadlbauer D;Strohmeier S;Nguyen THO;Chromikova V;McMahon M;Jiang K;Arunkumar GA;Jurczyszak D;Polanco J;Bermudez-Gonzalez M;Kleiner G;Aydillo T;Miorin L;Fierer DS;Lugo LA;Kojic EM;Stoever J;Liu STH;Cunningham-Rundles C;Felgner PL;Moran T;García-Sastre A;Caplivski D;Cheng AC;Kedzierska K;Vapalahti O;Hepojoki JM;Simon V;Krammer F
通讯作者：
Krammer F
影响因子：
9.4
作者：
TURNER, R;LATHEY, JL;BELSHE, RB
通讯作者：
BELSHE, RB
DOI：
10.3390/pathogens9100803
发表时间：
2020-09-28
期刊：
Pathogens (Basel, Switzerland)
影响因子：
--
作者：
Alandijany TA;El-Kafrawy SA;Tolah AM;Sohrab SS;Faizo AA;Hassan AM;Alsubhi TL;Othman NA;Azhar EI
通讯作者：
Azhar EI
中国武汉市 138 例 2019 新型冠状病毒感染肺炎住院患者的临床特征。
影响因子：
120.7
作者：
Wang, Dawei;Hu, Bo;Peng, Zhiyong
通讯作者：
Peng, Zhiyong