Single molecule localization imaging of exosomes using blinking silicon quantum dots
Single molecule localization imaging of exosomes using blinking silicon quantum dots
复制标题
使用闪烁硅量子点进行外泌体的单分子定位成像
DOI：
10.1088/1361-6528/aaa375
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发表时间：
2018-01
期刊：
影响因子：
3.5
通讯作者：
Cui Yiping
中科院分区：
文献类型：
--
作者：
Zong Shenfei;Zong Junzhu;Chen Chen;Jiang Xiaoyue;Zhang Yizhi;Wang Zhuyuan;Cui Yiping
发现新的适用于单分子定位显微镜(SMLM)的荧光团，对于推动SMLM在生物或材料科学中的应用具有重要意义。在这里，我们发现硅量子点(Si量子点)具有荧光闪烁行为，使它们成为SMLM的极佳候选者。利用一种简便的微波辅助方法制备了硅量子点。单粒子荧光测量证实了硅量子点的闪烁，其空间分辨率约为30 nm。为了探索硅量子点作为SMLM成像纳米探针的潜在应用，选择了尺寸较小(直径50-100 nm)的细胞来源的外切体作为研究对象。由于CD63通常存在于外切体的膜上，因此CD63适配子被连接到硅量子点的表面形成能够特异性识别外切体的纳米探针。SMLM成像表明，基于硅量子点的纳米探针确实可以实现对外切小体的超分辨光学成像。更重要的是，在水或PbS缓冲液中可以观察到硅量子点的闪烁，而不需要特殊的成像缓冲液。此外，考虑到硅具有高度的生物相容性，硅量子点的细胞毒性应该是最小的。这些特性使得硅量子点非常适合SMLM应用，特别是活体细胞成像。
Discovering new fluorophores, which are suitable for single molecule localization microscopy (SMLM) is important for promoting the applications of SMLM in biological or material sciences. Here, we found that silicon quantum dots (Si QDs) possess a fluorescence blinking behavior, making them an excellent candidate for SMLM. The Si QDs are fabricated using a facile microwave-assisted method. Blinking of Si QDs is confirmed by single particle fluorescence measurement and the spatial resolution achieved is about 30 nm. To explore the potential application of Si QDs as the nanoprobes for SMLM imaging, cell derived exosomes are chosen as the object owing to their small size (50–100 nm in diameter). Since CD63 is commonly presented on the membrane of exosomes, CD63 aptamers are attached to the surface of Si QDs to form nanoprobes which can specifically recognize exosomes. SMLM imaging shows that Si QDs based nanoprobes can indeed realize super resolved optical imaging of exosomes. More importantly, blinking of Si QDs is observed in water or PBS buffer with no need for special imaging buffers. Besides, considering that silicon is highly biocompatible, Si QDs should have minimal cytotoxicity. These features make Si QDs quite suitable for SMLM applications especially for live cell imaging.
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影响因子：
12.6
作者：
Wei, Fang;Yang, Jieping;Wong, David T. W.
通讯作者：
Wong, David T. W.
影响因子：
48
作者：
Dempsey, Graham T.;Vaughan, Joshua C.;Chen, Kok Hao;Bates, Mark;Zhuang, Xiaowei
通讯作者：
Zhuang, Xiaowei
影响因子：
6
作者：
D. Wöll;C. Flors
通讯作者：
D. Wöll;C. Flors
高级成像 内吞和细胞骨架动力学的扩展分辨率结构照明成像
DOI：
10.1126/science.aab3500
发表时间：
2015-08-28
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Li D;Shao L;Chen BC;Zhang X;Zhang M;Moses B;Milkie DE;Beach JR;Hammer JA 3rd;Pasham M;Kirchhausen T;Baird MA;Davidson MW;Xu P;Betzig E
通讯作者：
Betzig E
影响因子：
46.9
作者：
Ku, Taeyun;Swaney, Justin;Park, Jeong-Yoon;Albanese, Alexandre;Murray, Evan;Cho, Jae Hun;Park, Young-Gyun;Mangena, Vamsi;Chen, Jiapei;Chung, Kwanghun
通讯作者：
Chung, Kwanghun