Genetic diversity of the planthopper, Sogatella furcifera in the Greater Mekong Subregion detected by inter-simple sequence repeats (ISSR) markers.
Genetic diversity of the planthopper, Sogatella furcifera in the Greater Mekong Subregion detected by inter-simple sequence repeats (ISSR) markers.
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DOI：
10.1673/031.010.5201
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Li ZY
中科院分区：
文献类型：
--
作者：
Liu JN;Gui FR;Li ZY
白色背飞虱是亚洲水稻的重要害虫。本研究采用简单重复序列（inter-simple sequence repeat，ISSR）分子标记技术，对大湄公河次区域14个地区的47个种群的遗传多样性和分化进行了研究。共筛选出14条引物，扩增出121条清晰可辨的条带，平均每条引物扩增出8.6条条带。AMOVA分析表明，地理区域间的遗传变异（79.84%）高于区域内居群间的遗传变异（20.16%），FST值为0.72，表明遗传分化程度较高。对47个居群进行邻接聚类分析，结果表明，云南省西南部和缅甸居群为主要聚类群，云南省东南部和中部以及越南和老挝居群为主要聚类群。经Mantel检验，遗传距离与地理距离之间无显著正相关（r = 0.2230，p = 0.8448），表明地理隔离并没有影响到S. furcifera种群。为认识和推测S.并揭示了可用的信息，以制定管理这种远距离迁徙害虫的可持续战略。
The white backed planthopper, Sogatella furcifera (Hemiptera: Delphacidae), is a serious pest of rice in Asia. In the present study, inter-simple sequence repeat (ISSR) markers were employed to investigate the genetic diversity and differentiation of 47 populations sampled from 14 prefectures of the Greater Mekong Subregion. A total of 14 selected primers yielded 121 bright and discernible bands, with an average of 8.6 bands per primer. According to the hierarchical analysis of molecular variance (AMOVA), the genetic variation among geographic regions (79.84%) was higher than that of among populations within region (20.16%), and the FST value was 0.72, indicating a high level of genetic differentiation. Neighbor-Joining cluster analysis of the 47 populations showed two major clusters, one consisting of mostly southwestern Yunnan Province and Myanmar populations; and the other one consisting of southeastern and central of Yunnan Province plus Vietnam and Laos populations. No significant positive correlation was observed between genetic and geographic distances by Mantel test (r = 0.2230, p = 0.8448), indicating the role of geographic isolation did not shape the genetic structure of the sampled S. furcifera populations. This paper provides useful data for understanding and speculating the migration of S. furcifera and reveals available information to develop sustainable strategies for manage this long-range migratory pest.
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影响因子：
5
作者：
Nielsen, Jens C. O.
通讯作者：
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影响因子：
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1.5
作者：
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3.1
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