Microbial reporters of metal bioavailability.
Microbial reporters of metal bioavailability.
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DOI：
10.1111/j.1751-7915.2008.00022.x
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发表时间：
2008-07
影响因子：
5.7
通讯作者：
Belkin S
中科院分区：
文献类型：
--
作者：
Magrisso S;Erel Y;Belkin S
在试图评估环境污染的程度和影响时，通常不仅要量化所研究污染物的总浓度，还要量化其生物可利用部分：较高的生物可利用性，通常与流动性增加有关，意味着风险增加，但也可能促进生物修复。基因工程微生物可以通过可量化的信号对目标化学物质的存在做出反应，可以作为生物利用度评估的有力工具。本文综述了目前的知识，旨在测定金属生物利用度的微生物生物报告。在过去的15年中，已经开发了许多细菌金属传感器菌株，对广泛的环境重要金属目标表现出非常高的检测灵敏度。 这些构建体基于使用相对少量的基因启动子作为传感元件，以及更少的分子报告系统的选择;它们构成了一组潜在有用的工具，用于简单且具有成本效益的测定环境中重金属的生物利用度，以及定量污染物的非生物可利用部分。然而，尽管这些工具有其固有的优点，但它们还没有在真实的环境修复方案中实际用于金属生物利用度的评价。要做到这一点，监管机构的认可是必不可少的，测定条件的标准化也是如此。
When attempting to assess the extent and the implications of environmental pollution, it is often essential to quantify not only the total concentration of the studied contaminant but also its bioavailable fraction: higher bioavailability, often correlated with increased mobility, signifies enhanced risk but may also facilitate bioremediation. Genetically engineered microorganisms, tailored to respond by a quantifiable signal to the presence of the target chemical(s), may serve as powerful tools for bioavailability assessment. This review summarizes the current knowledge on such microbial bioreporters designed to assay metal bioavailability. Numerous bacterial metal‐sensor strains have been developed over the past 15 years, displaying very high detection sensitivities for a broad spectrum of environmentally significant metal targets. These constructs are based on the use of a relatively small number of gene promoters as the sensing elements, and an even smaller selection of molecular reporter systems; they comprise a potentially useful panel of tools for simple and cost‐effective determination of the bioavailability of heavy metals in the environment, and for the quantification of the non‐bioavailable fraction of the pollutant. In spite of their inherent advantages, however, these tools have not yet been put to actual use in the evaluation of metal bioavailability in a real environmental remediation scheme. For this to happen, acceptance by regulatory authorities is essential, as is a standardization of assay conditions.
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影响因子：
9.8
作者：
Darling, CTR;Thomas, VG
通讯作者：
Thomas, VG
影响因子：
8.9
作者：
Bontidean, L;Mortari, A;Csöregi, E
通讯作者：
Csöregi, E
影响因子：
4.9
作者：
Geebelen, W;Adriano, DC;Vangronsveld, J
通讯作者：
Vangronsveld, J
影响因子：
3.6
作者：
Cai, J;DuBow, MS
通讯作者：
DuBow, MS
影响因子：
4.5
作者：
Golding, GR;Kelly, CA;Barkay, T
通讯作者：
Barkay, T