Prevalence and temporal changes of mutations linked to antimalarial drug resistance in Plasmodium falciparum and Plasmodium vivax in Palawan, Philippines.
Prevalence and temporal changes of mutations linked to antimalarial drug resistance in Plasmodium falciparum and Plasmodium vivax in Palawan, Philippines.
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DOI：
10.1016/j.ijid.2021.12.318
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发表时间：
2022-03
期刊：
影响因子：
--
通讯作者：
Hafalla JCR
中科院分区：
文献类型：
--
作者：
Bareng APN;Grignard L;Reyes R;Fornace K;Spencer F;Macalinao ML;Luchavez J;Espino FE;Drakeley C;Hafalla JCR
对菲律宾的恶性疟原虫和间日疟原虫分离株进行了分析。在与抗疟药物耐药性相关的标记物中发现了不同的突变。没有一个突变是特别高的流行率。在过去的15年中，观察到这些突变的明确时间模式。pfcrt和pfmdr突变的减少与抗疟政策的改变一致。这项研究提供了2016年菲律宾巴拉望与抗疟药耐药性相关的关键单核苷酸多态性（SNP）流行率数据。研究结果与历史数据相结合，以模拟这些SNP在疟原虫分离株中流行的时间变化。使用耐药标志物pfmdr 1、pfcrt、pfdhfr、pfdhps、kelch-13、pvmdr 1、pvdhfr和pvdhps对疟原虫分离株进行基因分型。使用二项广义线性模型估计突变概率的时间趋势作为时间的函数。所有恶性疟原虫氯喹标记pfmdr 1和pfcrt测序的样本都有野生型等位基因。不同的突变模式观察到磺胺嘧啶/乙胺嘧啶标记pfdhps和pfdhfr，完整的五联体突变没有发现。没有观察到单核苷酸多态性青蒿素标记kelch-13。对于间日疟原虫，检测到pvmdr 1，pvdhfr和pvdhps的不同模式。研究结果表明，目前的药物仍然有效，该地区的耐药寄生虫进口和建立有限。明确的时间趋势得到认可，在过去15年中检测到的pfcrt和pfmdr突变比例显著下降，与抗疟药物政策的变化一致。持续监测抗疟药物耐药性对于支持消灭疟疾的努力十分重要。
Plasmodium falciparum and Plasmodium vivax isolates from the Philippines were analysed. Varying mutations were found in markers linked to resistance to antimalarial drugs. None of the mutations were particularly of high prevalence. Clear temporal patterns in these mutations were observed within the past 15 years. Decrease in pfcrt and pfmdr mutations are in line with antimalarial policy change. This study provides 2016 data on the prevalence of key single nucleotide polymorphisms (SNPs) associated with antimalarial drug resistance in Palawan, Philippines. Findings were combined with historical data to model temporal changes in the prevalence of these SNPs in Plasmodium isolates. Plasmodium isolates were genotyped using drug resistance markers pfmdr1, pfcrt, pfdhfr, pfdhps, kelch-13, pvmdr1, pvdhfr, and pvdhps. Temporal trends in the probability of mutations were estimated as a function of time using a binomial generalised linear model. All samples sequenced for Plasmodium falciparum chloroquine markers pfmdr1 and pfcrt had wild-type alleles. Varying mutation patterns were observed for the sulphadoxine/pyrimethamine markers pfdhps and pfdhfr; complete quintuplet mutations were not found. No SNPs were observed for the artemisinin marker kelch-13. For Plasmodium vivax, differing patterns were detected for pvmdr1, pvdhfr, and pvdhps. The study findings suggest that the current drugs remain effective and that there is limited importation and establishment of resistant parasites in the area. Clear temporal trends were recognised, with prominent decreases in the proportions of pfcrt and pfmdr mutations detected within the past 15 years, consistent with a change in antimalarial drug policy. Continuous surveillance of antimalarial drug resistance is important to support malaria elimination efforts.
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DOI：
10.4269/ajtmh.1963.12.840
发表时间：
1963-01-01
影响因子：
3.3
作者：
EYLES, DE;HOO, CC;SANDOSHAM, AA
通讯作者：
SANDOSHAM, AA
影响因子：
3.3
作者：
Biswas, S;Escalante, A;Lal, AA
通讯作者：
Lal, AA
影响因子：
4.9
作者：
Humphreys, G. S.;Merinopoulos, I.;Hallett, R. L.
通讯作者：
Hallett, R. L.
影响因子：
4.9
作者：
Chen, NH;Kyle, DE;Cheng, Q
通讯作者：
Cheng, Q
影响因子：
3
作者：
Dokunmu, Titilope M.;Adjekukor, Cynthia U.;Adebayo, Abiodun H.
通讯作者：
Adebayo, Abiodun H.