Caffeic Acid Phenethyl Ester (CAPE) Improves Boar Sperm Quality and Antioxidant Capacity in Liquid Preservation (17°C) Linked to AMPK Activity Maintenance.
Caffeic Acid Phenethyl Ester (CAPE) Improves Boar Sperm Quality and Antioxidant Capacity in Liquid Preservation (17°C) Linked to AMPK Activity Maintenance.
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DOI：
10.3389/fvets.2022.904886
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发表时间：
2022
影响因子：
3.2
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中科院分区：
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精液的液体保存是猪人工授精技术应用的关键。然而，精子的时间依赖性氧化损伤是液体保存期间的主要挑战之一。咖啡酸苯乙酯（CAPE）具有良好的抗氧化性能，并在与氧化应激相关的生殖器官损伤中具有潜在的治疗用途。腺苷一磷酸（AMP）激活的蛋白激酶（AMPK）参与调节细胞的氧化还原状态，对精子的保存有重要作用。在本研究中，我们首先评估了不同浓度的CAPE在液体储存过程中对精子质量的影响，以确定合适的添加量。为了进一步研究CAPE是否通过调节AMPK活性对公猪精子产生保护作用，在与H2 O2共孵育的条件下评价了精子质量参数、抗氧化能力和标记蛋白表达。结果表明，210 μmol/L CAPE处理的精子运动参数（总运动力和前向运动力）最高，功能完整性（线粒体活性、质膜完整性和顶体完整性）最好。即使在H2 O2存在的情况下，添加210 μmol/L CAPE不仅能显著改善精子质量参数，还能提高CAT、SOD和GSH-Px活性，增强精子抗氧化能力。此外，我们还发现，无论H_2O_2是否存在，CAPE都能影响AMPK、磷酸化AMPK α（p-AMPK）、SOD和Caspase-3的蛋白活性。本研究结果表明，CAPE在猪精子液态保存中具有潜在的应用价值，并初步表明CAPE对精子质量和抗氧化能力的改善可能是通过保护AMPK活性来实现的。需要进一步的研究来阐明CAPE减弱氧化应激介导的依赖于AMPK活性的损伤的具体机制。CAPE对猪精子保护作用的可能机制。CAPE诱导AMPK磷酸化，导致SOD 1的蛋白表达升高以消除ROS以及抑制Caspase-3的蛋白表达。提高了精液保存时间和精液质量。
Liquid preservation of boar sperm is crucial for artificial insemination application in pig production. However, time-dependent oxidative damage to sperm is one of the major challenges during the liquid preservation period. Caffeic acid phenethyl ester (CAPE) possesses excellent antioxidant properties and has potential therapeutic use in reproductive organ injury linked to oxidative stress. Adenosine monophosphate (AMP)-activated protein kinase (AMPK) involves in modulating the cellular redox state and exerts a beneficial effect on sperm preservation. In the present study, we firstly assessed different concentrations of CAPE that affect sperm quality during liquid storage to determine the appropriate addition. To further investigate whether CAPE exerts protective effects on boar sperm through modulation of AMPK activity, sperm quality parameters, antioxidant capacity, and marker protein expressions were evaluated under co-incubation with H2O2. The results showed that sperm treated with 210 μmol/L CAPE exhibited the highest motion parameters (total motility and progressive motility) and best functional integrity (mitochondrial activity, plasma membrane integrity, and acrosomal integrity). Even in the presence of H2O2, the addition of 210 μmol/L CAPE not only significantly improved sperm quality parameters, but also elevated CAT, SOD, and GSH-Px activities to enhance sperm antioxidant capacity. In addition, we found that CAPE could affect the protein activities of AMPK, phospho-AMPK α (p-AMPK), SOD, and Caspase-3 regardless of whether H2O2 is present or not. Our findings suggested that CAPE has potential application in liquid preservation of boar sperm and preliminary indicated that CAPE-induced improvement of sperm quality and antioxidant capacity should be mediated through conservation of AMPK activity. Further studies are required to illustrate the specific mechanism by which CAPE attenuates oxidative stress-mediated damages dependent on AMPK activity. The potential protective mechanism of CAPE on boar sperm. CAPE induces AMPK phosphorylation resulting in elevating the protein expression of SOD1 to eliminate ROS as well as inhibiting the protein expression of Caspase-3. Consequently, both the liquid preservation time and boar sperm quality are improved.
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DOI：
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发表时间：
2020-10-05
期刊：
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影响因子：
--
作者：
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影响因子：
20.1
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发表时间：
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影响因子：
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DOI：
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发表时间：
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期刊：
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影响因子：
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