Potential Use of Tannin Extracts as Additives in Semen Destined for Cryopreservation: A Review.
Potential Use of Tannin Extracts as Additives in Semen Destined for Cryopreservation: A Review.
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DOI：
10.3390/ani12091130
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发表时间：
2022-04-28
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用于人工生殖技术(ART)的精液冷冻会在冷冻和解冻过程中产生过多的活性氧(ROS)，从而影响精子细胞的存活率和活力。ROS在精子功能中起着生理作用，但受损的精子细胞产生过多的ROS会阻碍精子的活力及其使卵母细胞受精的能力。单宁是一类水溶性植物多酚，具有抗氧化和其他促进健康的作用，可以作为结合剂/受体来减少在冷冻和解冻过程中产生的过量ROS的有害影响。这篇综述首次分析了现有数据支持将单宁作为精液稀释剂的添加剂来提高冷冻精子在保存和解冻后的存活率。结论：单宁及其衍生物具有天然的保护作用，有可能改善冷冻后精子细胞的存活率。人工授精(AI)中精液的超低温保存会导致体内活性氧(ROS)的过度积累。这会导致精子在解冻后寿命缩短，活力降低，从而损害其功能。然而，某些水平的ROS对于促进精子获能是必不可少的，这是成功受精所必需的。单宁作为众所周知的抗氧化剂，可以作为ROS的结合剂/受体/清除剂来抑制ROS的破坏作用。本文分析了精液冷冻保存方案和单宁的保健功能，以及ROS对新鲜精液和冷冻精液寿命和受精率的影响。此外，我们调查了单宁提取物饲料补充剂对男性生育能力影响的现有证据。我们进一步询问了关于单宁作为精液稀释剂潜在添加剂的现有理论，它与精液质量的关系，以及现有理论在多大程度上被调查以开发可检验的新假说。重点介绍了单宁对ROS的影响，它们在冷冻保存过程中对精子结构和功能的调节作用，以及对解冻后精子活力、获能和受精能力的影响。单宁因其潜在的金属离子螯合、蛋白质沉淀和生物抗氧化能力而对生殖系统产生不同的影响。目前的数据首次支持进一步研究在精液稀释液中加入富含单宁的植物提取物，以提高冷冻精子在解冻后的存活率、活力和受精能力。
Freezing of semen used for artificial reproductive technologies (ART) affects the survival and vigour of sperm cells due to excessive production of reactive oxygen species (ROS) during the freezing and thawing processes. ROS plays a physiological role in sperm function but excessive ROS production from damaged sperm cells can hinder sperm’s motility and their ability to fertilise an oocyte. Tannins, a class of water-soluble plant polyphenols, are known to have antioxidant and other health-promoting effects and may serve as binders/acceptors to reduce the deleterious effects of excessive ROS produced during the freezing and thawing process. This review is the first to analyse the available data supporting the use of tannins as additives to semen extenders to improve the survival of cryopreserved spermatozoa during storage and after thawing. It is concluded that tannins and their derivatives have naturally protective properties with the potential to ameliorate sperm cell survival after freezing. Cryopreservation and storage of semen for artificial insemination (AI) result in excessive accumulation of reactive oxygen species (ROS). This leads to a shortened life span and reduced motility of spermatozoa post-thawing, with consequent impairment of their function. However, certain levels of ROS are essential to facilitate the capacitation of spermatozoa required for successful fertilisation. Tannins, as well-known antioxidant compounds, may act as ROS binders/acceptors/scavengers to inhibit the damaging effects of ROS. This review comprises an analysis of the semen cryopreservation protocol and health functions of tannins, as well as the effects of ROS on fresh and cryopreserved semen’s longevity and fertilisation. Additionally, we surveyed available evidence of the effects of tannin extract feed supplementation on male fertility. We furthermore interrogated existing theories on tannin use as a potential additive to semen extenders, its relationship with semen quality, and to what degree existing theories have been investigated to develop testable new hypotheses. Emphasis was placed on the effects of tannins on ROS, their involvement in regulating sperm structure and function during cryopreservation, and on post-thaw sperm motility, capacitation, and fertilising ability. The diverse effects of tannins on the reproductive system as a result of their potential metal ion chelation, protein precipitation, and biological antioxidant abilities have been identified. The current data are the first to support the further investigation of the incorporation of tannin-rich plant extracts into semen extenders to enhance the post-thaw survival, motility, and fertilising ability of cryopreserved spermatozoa.
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影响因子：
3.6
作者：
Aitken, RJ;Gordon, E;Irvine, DS
通讯作者：
Irvine, DS
影响因子：
6.1
作者：
Cerdá, B;Tomás-Barberán, FA;Espín, JC
通讯作者：
Espín, JC
影响因子：
2.8
作者：
Aurich, JE;Schonherr, U;Aurich, C
通讯作者：
Aurich, C
DOI：
10.1530/jrf.0.0830785
发表时间：
1988-07-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
KANTOLA, M;SAARANEN, M;VANHAPERTTULA, T
通讯作者：
VANHAPERTTULA, T
影响因子：
--
作者：
Aitken, RJ;Bennetts, LE;King, BV
通讯作者：
King, BV