The role of RANKL/RANK/OPG system in the canine model of hip periprosthetic infection osteolysis.
The role of RANKL/RANK/OPG system in the canine model of hip periprosthetic infection osteolysis.
复制标题
DOI：
10.5301/ijao.5000546
复制
发表时间：
2017-02-10
期刊：
影响因子：
--
通讯作者：
Song KG
中科院分区：
文献类型：
--
作者：
Zhang HW;Peng L;Li WB;Song KG
我们的目的是研究RANKL/RANK/OPG系统是否与假体周围骨质溶解伴感染性松动的发生率相关，并研究犬模型中正常和感染性松动髋关节假体周围滑液中RANKL/RANK/OPG系统表达的差异。12只健康成年杂种犬分为实验组和对照组。两组均在右侧行股骨头和股骨柄置换术。实验组关节腔内注射细菌液，对照组关节腔内注射等量生理盐水。分别于关节腔内注射细菌液后1、2、4、8、12、16、19周采集关节液标本，采用酶联免疫吸附试验（ELISA）检测RANKL/RANK/OPG系统的表达。所有动物的手术都很成功。由于手术感染或死亡，2只犬从结果分析中排除。关节液ELISA结果显示，试验组RANKL/OPG比值随时间呈显著上升趋势（p≤0.05），而对照组RANKL/OPG比值随时间变化缓慢（p>0.05）。试验组与对照组RANKL/OPG比值随时间呈明显上升趋势，但无统计学差异（p>0.05）。可以得出结论，RANKL/RANK/OPG系统与假体周围骨溶解伴感染性松动的发生率相关。因此，RANKL/RANK/OPG系统失衡与假体周围骨溶解伴感染性松动相关。
We aimed to investigate whether the RANKL/RANK/OPG system is associated with the incidence of periprosthetic osteolysis with septic loosening, and to investigate the differences of RANKL/RANK/OPG system expression in synovial fluid surrounding the normal and septic loosening hip prosthesis in canine models. Twelve healthy adult mongrel canines were divided into two groups: experimental and control. Femoral head and stem replacements were conducted on the right side in both groups. The experimental group received the bacteria fluid intra-articular injection and the other group received the same amount of saline in the same day. The synovial fluid samples were gathered at the 1st, 2nd, 4th, 8th, 12th, 16th and 19th week after the bacteria fluid intra-articular injection for enzyme-linked immunosorbent assay (ELISA), the expression of the RANKL/RANK/OPG system. Surgery on all animals was successful. Two dogs were excluded from the analysis of the result because of a surgery infection or death. The ELISA of the synovial fluid revealed that the ratio of RANKL/OPG showed a significant upward trend (p≤0.05) with time in the test group but the ratio of RANKL/OPG in the control group changed slowly over time (p>0.05). The ratio of RANKL/OPG value between the test and control group showed a significant upward trend, but had no statistical difference (p>0.05) over time. It could be concluded that the RANKL/RANK/OPG system is correlated with the incidence of periprosthetic osteolysis with septic loosening. Consequently, imbalance RANKL/RANK/OPG system was related to periprosthetic osteolysis with septic loosening.
登录
查看更多内容
影响因子：
4.1
作者：
Crotti TN;Dharmapatni AA;Alias E;Haynes DR
通讯作者：
Haynes DR
影响因子：
4.2
作者：
Bozic, Kevin J.;Ong, Kevin;Lau, Edmund;Kurtz, Steven M.;Vail, Thomas P.;Rubash, Harry E.;Berry, Daniel J.
通讯作者：
Berry, Daniel J.
影响因子：
4.5
作者：
Biscione, Fernando Martin;Couto, Renato Camargos;Pedrosa, Tania M. G.
通讯作者：
Pedrosa, Tania M. G.
在小鼠骨质溶解模型中，MMP-9 抑制通过下调 RANK/RANKL 抑制磨损碎片诱导的炎症性骨质溶解
影响因子：
5.4
作者：
Chen, Desheng;Zhang, Xianlong;Cheng, Tao
通讯作者：
Cheng, Tao
影响因子：
14
作者：
Masui, T;Sakano, S;Ishiguro, N
通讯作者：
Ishiguro, N