A single GATA factor plays discrete, lineage specific roles in ascidian heart development.
A single GATA factor plays discrete, lineage specific roles in ascidian heart development.
复制标题
单个 GATA 因子在海鞘心脏发育中发挥着离散的、谱系特定的作用。
DOI：
10.1016/j.ydbio.2011.01.007
复制
发表时间：
2011
影响因子：
2.7
通讯作者：
Davidson,Brad
中科院分区：
文献类型：
--
作者：
Ragkousi,Katerina;Beh,Jeni;Sweeney,Sarah;Starobinska,Ella;Davidson,Brad
加塔家族转录因子是脊椎动物心脏基因网络的核心组成部分。加塔因子还通过调节内胚层形态发生间接促进心脏形成。然而，加塔因子对脊椎动物心脏发生的精确影响被多个谱系内的功能冗余所掩盖。无脊椎动物脊索动物玻璃海鞘的早期心脏特化与脊椎动物相似，但只有一种加塔因子Ci-GATAa在心脏祖细胞和邻近的内胚层中表达。在这里，我们描绘了精确的，组织特异性的GATAa心脏形成的贡献。心脏祖细胞中GATAa活性的靶向抑制扰乱了它们的转录身份。内胚层GATAa功能的靶向抑制破坏内胚层形态发生。随后，双侧心脏祖细胞未能在腹侧中线融合。由此产生的表型与内胚层形态发生受到干扰时在脊椎动物胚胎中观察到的贲门裂惊人相似。这些发现表明，GATAa概括了细胞自主和非细胞自主的作用，由多个，冗余的加塔因子在脊椎动物心脏发生。
GATA family transcription factors are core components of the vertebrate heart gene network. GATA factors also contribute to heart formation indirectly through regulation of endoderm morphogenesis. However, the precise impact of GATA factors on vertebrate cardiogenesis is masked by functional redundancy within multiple lineages. Early heart specification in the invertebrate chordate Ciona intestinalis is similar to that of vertebrates but only one GATA factor, Ci-GATAa, is expressed in the heart progenitor cells and adjacent endoderm. Here we delineate precise, tissue specific contributions of GATAa to heart formation. Targeted repression of GATAa activity in the heart progenitors perturbs their transcriptional identity. Targeted repression of endodermal GATAa function disrupts endoderm morphogenesis. Subsequently, the bilateral heart progenitors fail to fuse at the ventral midline. The resulting phenotype is strikingly similar to cardia bifida, as observed in vertebrate embryos when endoderm morphogenesis is disturbed. These findings indicate that GATAa recapitulates cell-autonomous and non-cell-autonomous roles performed by multiple, redundant GATA factors in vertebrate cardiogenesis.
登录
查看更多内容
影响因子：
56.9
作者：
Christiaen, Lionel;Davidson, Brad;Levine, Michael
通讯作者：
Levine, Michael
影响因子：
4.6
作者：
M. Gannon;D. Bader
通讯作者：
M. Gannon;D. Bader
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
4.6
作者：
J. Reecy;Xuyang Li;M. Yamada;F. DeMayo;C. Newman;R. Harvey;R. Schwartz
通讯作者：
J. Reecy;Xuyang Li;M. Yamada;F. DeMayo;C. Newman;R. Harvey;R. Schwartz
影响因子：
2.7
作者：
NISHIDA, H
通讯作者：
NISHIDA, H