Nanodroplet real-time PCR system with laser assisted heating.
Nanodroplet real-time PCR system with laser assisted heating.
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DOI：
10.1364/oe.17.000218
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发表时间：
2009-01-05
期刊：
影响因子：
3.8
通讯作者：
Faris GW
中科院分区：
文献类型：
--
作者：
Kim H;Dixit S;Green CJ;Faris GW
We report the successful application of low-power (~30 mW) laser radiation as an optical heating source for high-speed real-time polymerase chain reaction (PCR) amplification of DNA in nanoliter droplets dispersed in an oil phase. Light provides the heating, temperature measurement, and Taqman real-time readout in nanoliter droplets on a disposable plastic substrate. A selective heating scheme using an infrared laser appears ideal for driving PCR because it heats only the droplet, not the oil or plastic substrate, providing fast heating and completing the 40 cycles of PCR in 370 seconds. No microheaters or microfluidic circuitry were deposited on the substrate, and PCR was performed in one droplet without affecting neighboring droplets. The assay performance was quantitative and its amplification efficiency was comparable to that of a commercial instrument.
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影响因子：
7.4
作者：
Slyadnev, MN;Tanaka, Y;Kitamori, T
通讯作者：
Kitamori, T
DOI：
10.1007/bf00273298
发表时间：
1972-01-01
期刊：
HUMANGENETIK
影响因子：
--
作者：
BROSS, K;KRONE, W
通讯作者：
KRONE, W
影响因子：
7.4
作者：
He, MY;Sun, CH;Chiu, DT
通讯作者：
Chiu, DT
影响因子：
6.1
作者：
Guttenberg, Z;Müller, H;Wixforth, A
通讯作者：
Wixforth, A
影响因子：
2.4
作者：
Baroud, Charles N.;Delville, Jean-Pierre;Wunenburger, Regis
通讯作者：
Wunenburger, Regis