Perinatal Exposure to Nicotine Alters Sperm RNA Profiles in Rats.
Perinatal Exposure to Nicotine Alters Sperm RNA Profiles in Rats.
复制标题
DOI：
10.3389/fendo.2022.893863
复制
发表时间：
2022
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
--
作者：
围产期吸烟与儿童哮喘有关，这是影响全球数百万儿童的最常见儿科疾病之一。令人感兴趣的是，这种疾病表型似乎是可遗传的，因为即使在后代没有持续接触吸烟的情况下，它也可以持续多代。尽管由围产期暴露于吸烟或尼古丁诱导的儿童哮喘的分子机制仍然难以捉摸，但已经提出了一种表观遗传机制，这得到了我们早期对生殖系DNA甲基化（5mC）和组蛋白标记（H3和H4乙酰化）的分析数据的支持。为了进一步研究围产期尼古丁暴露诱导的儿童哮喘的潜在表观遗传，我们分析了F1雄性大鼠精子中的大小RNA。我们的数据显示，围产期暴露于尼古丁会导致精子携带的RNA（包括mRNA和小RNA）谱的改变，而罗格列酮（一种PPARγ激动剂）可以减弱尼古丁的影响，并将F1雄性大鼠的精子携带的RNA谱逆转至接近安慰剂对照水平。
Perinatal exposure to smoking has been associated with childhood asthma, one of the most common pediatric conditions affecting millions of children globally. Of great interest, this disease phenotype appears heritable as it can persist across multiple generations even in the absence of persistent exposure to smoking in subsequent generations. Although the molecular mechanisms underlying childhood asthma induced by perinatal exposure to smoking or nicotine remain elusive, an epigenetic mechanism has been proposed, which is supported by the data from our earlier analyses on germline DNA methylation (5mC) and histone marks (H3 and H4 acetylation). To further investigate the potential epigenetic inheritance of childhood asthma induced by perinatal nicotine exposure, we profiled both large and small RNAs in the sperm of F1 male rats. Our data revealed that perinatal exposure to nicotine leads to alterations in the profiles of sperm-borne RNAs, including mRNAs and small RNAs, and that rosiglitazone, a PPARγ agonist, can attenuate the effect of nicotine and reverse the sperm-borne RNA profiles of F1 male rats to close to placebo control levels.
登录
查看更多内容
影响因子：
2.9
作者：
Gao X;Dai M;Li Q;Wang Z;Lu Y;Song Z
通讯作者：
Song Z
影响因子：
4.6
作者：
Ahlawat, Sonika;Arora, Reena;Vijh, Ramesh Kumar
通讯作者：
Vijh, Ramesh Kumar
影响因子：
30.8
作者：
Allen, M;Heinzmann, A;Cookson, WOCM
通讯作者：
Cookson, WOCM
影响因子：
9.6
作者：
Gibbs, Kevin;Collaco, Joseph M.;McGrath-Morrow, Sharon A.
通讯作者：
McGrath-Morrow, Sharon A.
影响因子：
12.4
作者：
Gervasini, G.;Agundez, J. A. G.;Garcia-Martin, E.
通讯作者：
Garcia-Martin, E.