Impact of resuscitation promoting factor (Rpf) in membrane bioreactor treating high-saline phenolic wastewater: Performance robustness and Rpf-responsive bacterial populations
Impact of resuscitation promoting factor (Rpf) in membrane bioreactor treating high-saline phenolic wastewater: Performance robustness and Rpf-responsive bacterial populations
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复苏促进因子 (Rpf) 对膜生物反应器处理高盐酚类废水的影响：性能鲁棒性和 Rpf 响应细菌群
DOI：
10.1016/j.cej.2018.09.197
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发表时间：
2019-02
影响因子：
15.1
通讯作者：
Sun Faqian
中科院分区：
文献类型：
--
作者：
Su Xiaomei;Wang Yuyang;Xue Binbing;Hashmi Muhammad Zaffar;Lin Hongjun;Chen Jianrong;Wang Zhen;Mei Rongwu;Sun Faqian
提高膜生物反应器（MBR）中细菌的活性是解决高盐含酚废水生物处理效率低的关键。预计藤黄微球菌复苏促进因子（Rpf）可提高细菌种群的降解性能。在这项研究中，一个控制MBR（c-MBR）和一个处理MBR（t-MBR）与Rpf的影响进行了评估。通过培养依赖性和培养非依赖性技术研究了响应Rpf添加的潜在细菌种群。结果表明，在高盐胁迫下，Rpf能显著提高苯酚的去除率。在MBR系统中，当进水酚浓度为1800 mg/L、NaCl浓度为30 g/L时，t-MBR对出水酚的去除率（221-272 mg/L）高于c-MBR（接近于零）。在苯酚初始浓度为1500 mg/L、NaCl浓度为60 g/L的条件下，t-MBR污泥在100 h内苯酚去除率接近100%，而c-MBR污泥的苯酚去除率仅为54.7%。高通量16 SrRNA分析表明，t-MBR中根瘤菌属的细菌大量富集。γ-变形杆菌中的假单胞菌属（PseudomonasinGammaproteobacteria）被鉴定为优势复苏细菌。这些结果表明，在高盐条件下，α-Proteobacteria和γ-Proteobacteria中的Rpf响应菌是提高苯酚降解能力的关键菌群。
Enhancement of bacterial activity in membrane bioreactor (MBR) is crucial to overcome the low efficiency of biological process for treating phenolic wastewater with high salinity. It is predicted that resuscitation promoting factor (Rpf) fromMicrococcus luteusmay improve the degradative performance of bacterial populations. In this study, a control MBR (c-MBR) and a treatment MBR (t-MBR) with Rpf addition were operated for assessing the influences of Rpf. The potential bacterial populations in response to Rpf addition were investigated by both culture-dependent and culture-independent techniques. Results showed that Rpf significantly enhanced phenol removal under high salinity stress. In the MBR system, a higher efficiency for phenol removal in the effluent was observed in t-MBR compared to that in c-MBR (nearly zero versus 221–272 mg/L) under phenol concentration of 1800 mg/L and 30 g/L NaCl in the influent. Batch tests further demonstrated that, at the initial phenol concentration of 1500 mg/L and 60 g/L NaCl, sludge from t-MBR could achieve almost 100% phenol removal within 100 h, while it was only 54.7% for the sludge from c-MBR. High-throughput 16S rRNA analysis indicated that the genusRhizobiuminAlphaproteobacteriawas greatly enriched in the t-MBR. The genusPseudomonasinGammaproteobacteriawas identified as the dominant resuscitated bacteria. These findings suggested that Rpf-responsive bacterial species belonging toAlphaproteobacteriaandGammaproteobacteriawere key populations contributed to better phenol-degrading capabilities under high salinity condition.
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