Transcranial photoacoustic characterization of neurovascular physiology during early-stage photothrombotic stroke in neonatal pigletsin vivo.
Transcranial photoacoustic characterization of neurovascular physiology during early-stage photothrombotic stroke in neonatal pigletsin vivo.
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DOI：
10.1088/1741-2552/ac4596
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发表时间：
2022-01-05
影响因子：
4
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中科院分区：
文献类型：
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据估计，围产期缺血性脑卒中发生率为1/2300-1/5000活产婴儿，但从整体缺氧缺血中进行早期鉴别诊断往往很困难。在这项研究中，我们测试了手持式经颅光声（PA）成像探针在脑卒中发作2小时内无创检测局灶性光血栓性卒中（PTS）的能力。在麻醉的新生仔猪中，通过光/染料PTS技术随机植入17个约1 cm2面积的脑卒中病变（n = 11）。对侧非缺血区作为对照组织，对PA血红蛋白指标进行区分对比：氧饱和度、总血红蛋白（tHb）、含氧和脱氧血红蛋白（HbO2和HbR）的个体数量。2小时pa来源的组织氧饱和度在对照组和受影响的感兴趣区域之间产生了显著的分离（p < 0.0001），这与卒中后24小时脑梗死在三苯四唑氯染色图像中证实的结果很好地匹配。HbO2的数量也显示出显著的差异（p = 0.021），而tHb和HbR没有显著的差异。患者工作特征曲线分析和多变量数据分析也与上述结果一致。本研究表明，手持式经颅PA神经成像装置可以检测新生儿仔猪大脑皮层的区域性血栓性卒中。特别是，我们得出结论，血氧饱和度指标可以单独用于识别局部脑卒中病变。tHb缺乏变化可能与任意手持成像配置和/或血栓性卒中中红细胞的夹持有关。
Perinatal ischemic stroke is estimated to occur in 1/2300–1/5000 live births, but early differential diagnosis from global hypoxia-ischemia is often difficult. In this study, we tested the ability of a hand-held transcranial photoacoustic (PA) imaging probe to non-invasively detect a focal photothrombotic stroke (PTS) within 2 h of stroke onset in a gyrencephalic piglet brain. About 17 stroke lesions of approximately 1 cm2 area were introduced randomly in anterior or posterior cortex via the light/dye PTS technique in anesthetized neonatal piglets (n = 11). The contralateral non-ischemic region served as control tissue for discrimination contrast for the PA hemoglobin metrics: oxygen saturation, total hemoglobin (tHb), and individual quantities of oxygenated and deoxygenated hemoglobin (HbO2 and HbR). The PA-derived tissue oxygen saturation at 2 h yielded a significant separation between control and affected regions-of-interest (p < 0.0001), which were well matched with 24 h post-stroke cerebral infarction confirmed in the triphenyltetrazolium chloride-stained image. The quantity of HbO2 also displayed a significant contrast (p = 0.021), whereas tHb and HbR did not. The analysis on receiver operating characteristic curves and multivariate data analysis also agreed with the results above. This study shows that a hand-held transcranial PA neuroimaging device can detect a regional thrombotic stroke in the cerebral cortex of a neonatal piglet. In particular, we conclude that the oxygen saturation metric can be used alone to identify regional stroke lesions. The lack of change in tHb may be related to arbitrary hand-held imaging configuration and/or entrapment of red blood cells within the thrombotic stroke.
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DOI：
10.2147/dddt.s56071
发表时间：
2015
期刊：
Drug design, development and therapy
影响因子：
--
作者：
Fluri F;Schuhmann MK;Kleinschnitz C
通讯作者：
Kleinschnitz C
影响因子：
1.9
作者：
Harbert MJ;Tam EW;Glass HC;Bonifacio SL;Haeusslein LA;Barkovich AJ;Jeremy RJ;Rogers EE;Glidden DV;Ferriero DM
通讯作者：
Ferriero DM
影响因子：
7.2
作者：
Adami RR;Grundy ME;Poretti A;Felling RJ;Lemmon M;Graham EM
通讯作者：
Graham EM
DOI：
10.3389/fnene.2010.00010
发表时间：
2010
期刊：
Frontiers in neuroenergetics
影响因子：
--
作者：
Hu S;Wang LV
通讯作者：
Wang LV
影响因子：
3.6
作者：
Gunn, AJ;Bennet, L;Gunn, TR
通讯作者：
Gunn, TR