Gene expression profiling of naïve sheep genetically resistant and susceptible to gastrointestinal nematodes.
Gene expression profiling of naïve sheep genetically resistant and susceptible to gastrointestinal nematodes.
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天真的绵羊的基因表达分析在遗传上具有抗性，并且对胃肠道线虫敏感。
DOI：
10.1186/1471-2164-7-42
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发表时间：
2006-03-06
期刊：
影响因子：
4.4
通讯作者：
McEwan, JC
中科院分区：
文献类型：
--
作者：
Keane, OM;Zadissa, A;Wilson, T;Hyndman, DL;Greer, GJ;Baird, DB;McCulloch, AF;Crawford, AM;McEwan, JC
胃肠道线虫是放牧反刍动物发病和死亡的主要原因。然而，已知个别动物或品种对感染的抵抗力不同。基因表达谱分析使我们能够同时检查大量的转录本，以确定那些有助于动物的易感性或耐药性的转录本。为了鉴定在绵羊对胃肠道线虫的遗传抗性和易感性之间具有差异表达模式的基因，构建了20，000个点的绵羊cDNA微阵列。该阵列被用来询问9，238个已知基因在四只抗性和四只易感雌性羔羊的十二指肠组织中的表达。使用幼稚动物以观察在没有胃肠道线虫感染的情况下差异表达的基因。鉴定了41个独特的已知基因，这些基因在抗性动物和易感动物之间差异表达。选择的基因的北方印迹证实差异表达。差异表达的基因具有多种功能，尽管许多与应激反应和刺激反应相关的基因在易感动物中表达更高。我们构建了第一个报道的绵羊微阵列，并使用该阵列检测了对胃肠道线虫感染具有遗传抗性和易感性的羔羊的基因表达。这项研究表明，易感动物似乎对非线虫攻击产生超敏感的免疫反应。因此，即使在没有胃肠道线虫的情况下，易感动物的胃肠道也会受到压力和损害。这些因素可能导致这些动物的遗传易感性。
Gastrointestinal nematodes constitute a major cause of morbidity and mortality in grazing ruminants. Individual animals or breeds, however, are known to differ in their resistance to infection. Gene expression profiling allows us to examine large numbers of transcripts simultaneously in order to identify those transcripts that contribute to an animal's susceptibility or resistance. With the goal of identifying genes with a differential pattern of expression between sheep genetically resistant and susceptible to gastrointestinal nematodes, a 20,000 spot ovine cDNA microarray was constructed. This array was used to interrogate the expression of 9,238 known genes in duodenum tissue of four resistant and four susceptible female lambs. Naïve animals were used in order to look at genes that were differentially expressed in the absence of infection with gastrointestinal nematodes. Forty one unique known genes were identified that were differentially expressed between the resistant and susceptible animals. Northern blotting of a selection of the genes confirmed differential expression. The differentially expressed genes had a variety of functions, although many genes relating to the stress response and response to stimulus were more highly expressed in the susceptible animals. We have constructed the first reported ovine microarray and used this array to examine gene expression in lambs genetically resistant and susceptible to gastrointestinal nematode infection. This study indicates that susceptible animals appear to be generating a hyper-sensitive immune response to non-nematode challenges. The gastrointestinal tract of susceptible animals is therefore under stress and compromised even in the absence of gastrointestinal nematodes. These factors may contribute to the genetic susceptibility of these animals.
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影响因子：
2.2
作者：
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通讯作者：
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