Identification of cis and trans-elements involved in the timed control of a Caulobacter flagellar gene.
Identification of cis and trans-elements involved in the timed control of a Caulobacter flagellar gene.
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鉴定参与柄杆菌鞭毛基因定时控制的顺式和反式元件。
DOI：
10.1016/0022-2836(91)90539-i
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发表时间：
1991
影响因子：
5.6
通讯作者：
Shapiro,L
中科院分区：
文献类型：
--
作者：
Gober,JW;Xu,H;Dingwall,AK;Shapiro,L
编码月牙鞭毛杆菌结构组分的基因是暂时受控的，它们的表达顺序反映了组装的顺序。包含鞭毛钩蛋白结构基因的操纵子的转录发生在细胞周期的特定时间，转录所需的信息包含在转录起始位点- 81和- 120碱基对之间的区域内。为了确定对钩子操纵子转录的时间控制有贡献的序列元件，我们在5 '区构建了缺失和碱基变化，并将突变的调控区域融合到转录报告基因上。我们证明了转录起始位点的序列3 '与时间控制无关。我们确认- 81和- 120碱基对之间的上游序列是时间激活所必需的，转录也需要- 26到- 46碱基对的序列。- 81和- 122碱基对之间区域的特异性结合活性被证明是暂时控制的，出现在钩子操纵子转录激活之前。这种结合活性在含有突变inflaoa和defect的菌株中缺失，这两个基因位于鞭毛结构的顶端，已知是钩子操纵子转录所必需的。因此，钩子操纵子上游区域包含一个响应暂时受控的反式作用因子的序列元件，并与第二个序列元件一起导致转录的定时激活。
The genes encoding the structural components of theCaulobacter crescentusflagellum are temporally controlled and their order of expression reflects the sequence of assembly. Transcription of the operon containing the structural gene for the flagellar hook protein occurs at a defined time in the cell cycle, and information necessary for transcription is contained within a region between −81 and −120 base-pairs from the transcription start site. To identify the sequence elements that contribute to the temporal control of hook operon transcription, we constructed deletions and base changes in the 5′ region and fused the mutagenized regulatory region to transcription reporter genes. We demonstrate that sequences 3′ to the transcription start site do not contribute to temporal control. We confirm that upstream sequences between −81 and −120 base-pairs are necessary for temporal activation, and that transcription also requires sequences at −26 to −46 base-pairs. A specific binding activity for the region between −81 and −122 base-pairs was shown to be temporally controlled, appearing prior to the activation of hook operon transcription. This binding activity was missing from strains containing mutations inflaOandflaW, two genes near the top of the flagellar hierarchy known to be required for hook operon transcription. Thus, the hook operon upstream region contains a sequence element that responds to a temporally controlledtrans-acting factor(s), and in concert with a second sequence element causes the timed activation of transcription.
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柄杆菌鞭毛和趋化基因的级联调节。
影响因子：
5.6
作者：
Champer,R;Dingwall,A;Shapiro,L
通讯作者：
Shapiro,L
新月柄杆菌中特定膜蛋白的合成是 DNA 复制和磷脂合成的功能。
影响因子：
5.6
作者：
Shapiro,L;Mansour,J;Shaw,P;Henry,S
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影响因子：
5.6
作者：
Mullin,D;Minnich,S;Chen,LS;Newton,A
通讯作者：
Newton,A
影响因子：
3.2
作者：
CONTRERAS, I;SHAPIRO, L;HENRY, S
通讯作者：
HENRY, S
影响因子：
10.5
作者：
KROOS, L;KAISER, D
通讯作者：
KAISER, D