Lymphoid tissue ontogeny in the mummichog, Fundulus heteroclitus.
Lymphoid tissue ontogeny in the mummichog, Fundulus heteroclitus.
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DOI：
10.1002/ar.20740
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发表时间：
2008-10
影响因子：
2
通讯作者：
Rice, Charles D.
中科院分区：
文献类型：
--
作者：
Hunt, Laura R.;Rice, Charles D.
大多数检查硬骨鱼类淋巴器官发育的个体发生的研究使用水产养殖或遗传研究感兴趣的物种，迄今为止主要集中在严格的海洋或严格的淡水物种上。mummichog, Fundulus heteroclitus，也被称为河口鳉鱼，是研究发育免疫生物学的独特模型，因为它是全盐性的，具有高度的耐热性，并且具有独特的繁殖策略。研究了干尸的胚胎和幼虫淋巴组织发育的个体发生。第一个出现的淋巴器官是头肾，第一个出现的是脾，第一个出现的是胸腺。对rag1基因进行了部分克隆和测序，结果显示其在其他脊椎动物rag1基因中高度保守。使用QT-PCR监测rag1的时间表达，结果显示rag1在3和4 wph时达到最大强度，然后下降到2 wph前的水平。总的来说，这项研究表明幼mummichos直到5-wph后的一段时间才具备T细胞或b细胞反应的能力。尽管河口鳉鱼能耐受各种盐度，但其淋巴组织的发育模式与已报道的严格意义上的海洋（窄盐）硬骨鱼相似。因此，mummichog应该是了解大多数海洋硬骨鱼发育免疫生物学的一个方便的模型。
Most studies that examine the ontogeny of lymphoid organ development in teleostean fishes use species of interest to aquaculture or genetic research, and to date have focused on strictly marine or strictly freshwater species. The mummichog, Fundulus heteroclitus, also known as the estuarine killifish, is a unique model for studies on developmental immunobiology because it is euryhaline, has a high degree of thermal tolerance, and has a unique reproductive strategy. Embryonic and larval mummichogs were examined for the ontogeny of lymphoid tissue development. The first lymphoid organ to appear was the head kidney at 1 dph, followed by the spleen at 1 wph, and then the thymus at 3 wph. Rag-1 was partially cloned and sequenced and shown to be highly conserved among other vertebrate Rag-1 genes. Using QT-PCR to monitor the temporal expression of Rag-1 it was shown to reach a maximum intensity at 3- and 4-wph, and to then drop to pre-2 wph levels. Overall, this study suggests that juvenile mummichogs do not possess the ability to mount T- or B-cell responses until some time after 5-wph. Even though the estuarine killifish tolerates a wide range of salinities, the developmental patterns of lymphoid tissues are similar to what has been reported for strictly marine (stenohaline) teleosts. Thus, the mummichog should be a convenient model for understanding the developmental immunobiology of most marine teleosts.
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