Differentiation of mesenchymal stem cells into nucleus pulposus cells in vitro
Differentiation of mesenchymal stem cells into nucleus pulposus cells in vitro
复制标题
间充质干细胞体外分化为髓核细胞
DOI：
10.1007/s11596-008-0210-x
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发表时间：
2008-05
期刊：
影响因子：
--
通讯作者：
Tao, Fenghua
中科院分区：
文献类型：
--
作者：
Li, Feng;Pan, Feng;Li, Guanghui;Tao, Fenghua
为寻找构建组织工程化椎间盘的新种子细胞来源，从新西兰白色兔中分离髓核细胞和间充质干细胞。将髓核细胞群体荧光标记并与MSC在有或没有直接接触的情况下共培养。每12 h观察一次形态学变化。半定量逆转录-聚合酶链反应进行评估的表达水平的Sox-9，aggreacan和II型胶原蛋白后，每24小时共培养。直接接触处理的MSC变圆并呈现环状外观。细胞直接接触共培养24 h后，标记基因的表达显著增加。在无直接接触的情况下，共培养后未发现显著变化。骨髓间充质干细胞与髓核细胞直接接触共培养是获得大量用于细胞基组织工程治疗的髓核细胞的可靠方法。
To find a new source of seed cells for constructing tissue-engineered intervertebral disc, nucleus pulposus (NP) cells and mesenchymal stem cells (MSCs) were isolated from New Zealand white rabbits. The nucleus pulposus cells population was fluorescence-laelled and co-cultured with MSCs with or without direct contact. Morphological changes were observed every 12 h. Semi-quantitative reverse transcriptase-polymerase chain reaction was performed to assess the expression levels of Sox-9, aggreacan and type II collagen every 24 h after the co-culture. MSCs treated with direct contact rounded up and presented a ring-like appearance. The expression of marker genes was significantly increased when cells were co-cultured with direct contact for 24 h. No significant change was found after coculture without direct contact. Co-culture of NP cells and MSCs with direct contact is a reliable method for generating large amount of NP cells used for cell-based tissue engineering therapy.
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影响因子：
3.3
作者：
Ding, Dah-Ching;Shyu, Woei-Cherng;Lin, Shinn-Zong
通讯作者：
Lin, Shinn-Zong
影响因子：
3
作者：
BUCKWALTER, JA
通讯作者：
BUCKWALTER, JA
影响因子：
10
作者：
S. Rankin
通讯作者：
S. Rankin
影响因子：
2.8
作者：
Caplan AI
通讯作者：
Caplan AI
DOI：
10.1016/b978-0-12-801888-0.00011-4
发表时间：
2016
期刊：
--
影响因子：
--
作者：
A. Patel;C. Bartlett;T. Ichim
通讯作者：
A. Patel;C. Bartlett;T. Ichim