Non-invasive detection of severe neutropenia in chemotherapy patients by optical imaging of nailfold microcirculation.
Non-invasive detection of severe neutropenia in chemotherapy patients by optical imaging of nailfold microcirculation.
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DOI：
10.1038/s41598-018-23591-0
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发表时间：
2018-03-28
影响因子：
4.6
通讯作者：
Castro-González C
中科院分区：
文献类型：
--
作者：
Bourquard A;Pablo-Trinidad A;Butterworth I;Sánchez-Ferro Á;Cerrato C;Humala K;Fabra Urdiola M;Del Rio C;Valles B;Tucker-Schwartz JM;Lee ES;Vakoc BJ;Padera TP;Ledesma-Carbayo MJ;Chen YB;Hochberg EP;Gray ML;Castro-González C
白细胞（WBC）评估被用于无数的临床程序作为免疫状态的一个指标。目前，通过全血样品的临床实验室分析获得WBC测定。血液的提取及其分析都限制了测量的可及性和频率。在这项研究中，我们证明了一种非侵入性设备在不需要抽血的情况下进行即时WBC分析的可行性，重点是患者的嗜中性粒细胞（最常见的WBC类型）变得非常低的化疗环境。特别是，我们建立了一个便携式光学原型，并使用它来收集22个微循环视频数据集从11个化疗患者。基于这些视频，我们确定了红细胞流动中移动的光学吸收间隙，将其用作白细胞通过甲襞毛细血管运动的代理。然后，我们发现，计算这些差距可以区分严重中性粒细胞减少症（每微升<500个中性粒细胞）的病例，与非中性粒细胞减少症病例（每微升> 1，500个中性粒细胞）相关的危及生命的感染风险增加。这一结果表明，光学成像，消费电子产品和数据分析的整合可以使患者无创筛查严重中性粒细胞减少症。更一般地说，这项工作为实现非侵入性WBC计数的长期目标迈出了第一步。
White-blood-cell (WBC) assessment is employed for innumerable clinical procedures as one indicator of immune status. Currently, WBC determinations are obtained by clinical laboratory analysis of whole blood samples. Both the extraction of blood and its analysis limit the accessibility and frequency of the measurement. In this study, we demonstrate the feasibility of a non-invasive device to perform point-of-care WBC analysis without the need for blood draws, focusing on a chemotherapy setting where patients’ neutrophils—the most common type of WBC—become very low. In particular, we built a portable optical prototype, and used it to collect 22 microcirculatory-video datasets from 11 chemotherapy patients. Based on these videos, we identified moving optical absorption gaps in the flow of red cells, using them as proxies to WBC movement through nailfold capillaries. We then showed that counting these gaps allows discriminating cases of severe neutropenia (<500 neutrophils per µL), associated with increased risks of life-threatening infections, from non-neutropenic cases (>1,500 neutrophils per µL). This result suggests that the integration of optical imaging, consumer electronics, and data analysis can make non-invasive screening for severe neutropenia accessible to patients. More generally, this work provides a first step towards a long-term objective of non-invasive WBC counting.
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DOI：
10.1093/bioinformatics/btu080
发表时间：
2014-06-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Maška M;Ulman V;Svoboda D;Matula P;Matula P;Ederra C;Urbiola A;España T;Venkatesan S;Balak DM;Karas P;Bolcková T;Streitová M;Carthel C;Coraluppi S;Harder N;Rohr K;Magnusson KE;Jaldén J;Blau HM;Dzyubachyk O;Křížek P;Hagen GM;Pastor-Escuredo D;Jimenez-Carretero D;Ledesma-Carbayo MJ;Muñoz-Barrutia A;Meijering E;Kozubek M;Ortiz-de-Solorzano C
通讯作者：
Ortiz-de-Solorzano C
影响因子：
3.1
作者：
Huang, Tzung-Chi;Lin, Wen-Chen;Lin, Kang-Ping
通讯作者：
Lin, Kang-Ping
影响因子：
5.5
作者：
Mugii, Naoki;Hasegawa, Minoru;Takehara, Kazuhiko
通讯作者：
Takehara, Kazuhiko
影响因子：
5.7
作者：
Berkow, Lauren;Rotolo, Stephanie;Mirski, Erin
通讯作者：
Mirski, Erin
影响因子：
8.8
作者：
Coquin, Julien;Bertarrex, Amandine;Ouattara, Alexandre
通讯作者：
Ouattara, Alexandre