Asymmetric distribution of Echinoid defines the epidermal leading edge during Drosophila dorsal closure.
Asymmetric distribution of Echinoid defines the epidermal leading edge during Drosophila dorsal closure.
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DOI：
10.1083/jcb.201009022
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发表时间：
2011-01-24
期刊：
影响因子：
--
通讯作者：
Nilson LA
中科院分区：
文献类型：
--
作者：
Laplante C;Nilson LA
当在对抗组织中失去结合伙伴时，亲同性细胞粘附蛋白Echinoid采用平面极化定位，促进平面细胞极性蛋白Bazooka/Par-3的平面极化定位，并将肌动球蛋白索组件定向到表皮前沿，从而确立了发育中的表皮的迁移方向。在黑腹果蝇的背侧闭合过程中，胚胎表皮的外侧片在其前缘形成一条肌动球蛋白索，并在羊膜上向背侧移动，在背侧中线处汇合。我们发现，在羊膜背关闭之前，亲同性细胞粘附分子Echinoid （Ed）从羊膜上消失，消除了与相邻的背最表皮（DME）细胞的亲同性相互作用，这些细胞包括前缘。在DME细胞中，Ed的平面极化分布对于肌动蛋白调节因子的局部积累、肌动球蛋白在前沿的索状形成以及支架蛋白Bazooka/PAR-3的极化定位至关重要。具有均匀Ed的二甲醚细胞不能组装成电缆并向背侧突出，表明电缆限制了背侧迁移。Ed在二甲醚细胞中的平面极化分布为二甲醚细胞肌动蛋白细胞骨架的极化提供了空间线索，定义了表皮前缘并建立了其收缩特性。
Upon loss of a binding partner in apposed tissue, the homophilic cell adhesion protein Echinoid adopts a planar polarized localization, which promotes the planar polarized localization of the planar cell polarity protein Bazooka/Par-3 and targets actomyosin cable assembly to the epidermal leading edge, thus establishing the migration direction of the developing epidermis. During Drosophila melanogaster dorsal closure, lateral sheets of embryonic epidermis assemble an actomyosin cable at their leading edge and migrate dorsally over the amnioserosa, converging at the dorsal midline. We show that disappearance of the homophilic cell adhesion molecule Echinoid (Ed) from the amnioserosa just before dorsal closure eliminates homophilic interactions with the adjacent dorsal-most epidermal (DME) cells, which comprise the leading edge. The resulting planar polarized distribution of Ed in the DME cells is essential for the localized accumulation of actin regulators and for actomyosin cable formation at the leading edge and for the polarized localization of the scaffolding protein Bazooka/PAR-3. DME cells with uniform Ed fail to assemble a cable and protrude dorsally, suggesting that the cable restricts dorsal migration. The planar polarized distribution of Ed in the DME cells thus provides a spatial cue that polarizes the DME cell actin cytoskeleton, defining the epidermal leading edge and establishing its contractile properties.
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影响因子：
2.5
作者：
Laplante, Caroline;Paul, Sarah M.;Nilson, Laura A.
通讯作者：
Nilson, Laura A.
影响因子：
4.6
作者：
Gates, Julie;Mahaffey, James P.;Peifer, Mark
通讯作者：
Peifer, Mark
影响因子：
4.6
作者：
Homem, Catarina C. F.;Peifer, Mark
通讯作者：
Peifer, Mark
影响因子：
4.6
作者：
Laplante, Caroline;Nilson, Laura A.
通讯作者：
Nilson, Laura A.
DOI：
10.1083/jcb.149.2.471
发表时间：
2000-04-17
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kiehart DP;Galbraith CG;Edwards KA;Rickoll WL;Montague RA
通讯作者：
Montague RA