Steady-state methadone blocks cocaine seeking and cocaine-induced gene expression alterations in the rat brain.
Steady-state methadone blocks cocaine seeking and cocaine-induced gene expression alterations in the rat brain.
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DOI：
10.1016/j.euroneuro.2008.09.004
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发表时间：
2009-04
影响因子：
5.6
通讯作者：
Kreek, Mary Jeanne
中科院分区：
文献类型：
--
作者：
Leri, Francesco;Zhou, Yan;Goddard, Benjamin;Levy, AnneMarie;Jacklin, Derek;Kreek, Mary Jeanne
为了阐明稳态美沙酮暴露对可卡因条件性刺激的反应以及可卡因引起的中枢阿片、下丘脑肌醇/食欲素和D2受体系统的改变的影响，雄性SD大鼠静脉注射1 mg/kg/inf可卡因，并配合视听刺激，持续3天。然后，植入微型泵释放载体或美沙酮30 mg/kg/d(SC)，在无可卡因和可卡因注射(20 mg/kg，ip)后评估杠杆按压刺激。结果发现，使用溶剂而不使用美沙酮的大鼠对可卡因的条件刺激有反应，伏隔核和杏仁基底外侧核的MU阿片受体基因表达升高，下丘脑外侧核的下丘脑下丘脑中的下丘脑/食欲素受体基因表达降低，尾壳核中的D2受体基因表达降低。这是第一次证明在可卡因暴露后给予稳定状态的美沙酮可以阻止可卡因诱导的行为和神经适应。
To elucidate the effects of steady-state methadone exposure on responding to cocaine conditioned stimuli and on cocaine-induced alterations in central opioid, hypocretin/orexin, and D2 receptor systems, male Sprague-Dawley rats received intravenous infusions of 1 mg/kg/inf cocaine paired with an audiovisual stimulus over three days of conditioning. Then, mini pumps releasing vehicle or 30 mg/kg/day methadone were implanted (SC), and lever pressing for the stimulus was assessed in the absence of cocaine and after a cocaine prime (20 mg/kg, IP). It was found that rats treated with vehicle, but not methadone, responded for the cocaine conditioned stimulus and displayed elevated mu-opioid receptor mRNA expression in the nucleus accumbens core and basolateral amygdala, reduced hypocretin/orexin mRNA in the lateral hypothalamus, and reduced D2 receptor mRNA in the caudate-putamen. This is the first demonstration that steady-state methadone administered after cocaine exposure blocks cocaine-induced behavioral and neural adaptations.
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影响因子：
3.8
作者：
CALCAGNETTI, DJ;SCHECHTER, MD
通讯作者：
SCHECHTER, MD
影响因子：
16.2
作者：
Borgland, SL;Taha, SA;Bonci, A
通讯作者：
Bonci, A
DOI：
10.1073/pnas.0507480102
发表时间：
2005-12-27
影响因子：
11.1
作者：
Boutrel, B;Kenny, PJ;de Lecea, L
通讯作者：
de Lecea, L
影响因子：
3.4
作者：
Capriles, N;Rodaros, D;Stewart, J
通讯作者：
Stewart, J
DOI：
10.1016/0169-328x(92)90178-e
发表时间：
1992-07-01
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
BRANCH, AD;UNTERWALD, EM;KREEK, MJ
通讯作者：
KREEK, MJ