Endogenous voltage gradients as mediators of cell-cell communication: strategies for investigating bioelectrical signals during pattern formation.
Endogenous voltage gradients as mediators of cell-cell communication: strategies for investigating bioelectrical signals during pattern formation.
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DOI：
10.1007/s00441-012-1329-4
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发表时间：
2013-04
影响因子：
3.6
通讯作者：
Levin, Michael
中科院分区：
文献类型：
--
作者：
Adams, Dany S.;Levin, Michael
除了众所周知的细胞-细胞通讯的化学模式外，我们还发现了一个重要而强大的生物电信号系统：由离子通道、泵和间隙连接驱动的质膜静息电压电位（Vmem）的变化。所有细胞中缓慢的Vmem变化是一种高度保守的信息传递途径，调节细胞增殖、迁移和分化。在胚胎和再生模式的形成以及肿瘤的破坏中，生物电信号作为大尺度解剖极性、器官身份和位置信息的中介。最近的发展已经产生了能够对这些生物物理信号及其与上游和下游典型遗传途径的联系进行高分辨率分析的工具。在这里，我们概述了生物电信号的研究，重点是使用分子生理学和发育遗传学来探索生物电事件在功能上的作用的最新方法。我们强调了逻辑、策略和发达的技术，任何研究人员都可以在他们自己的工作中使用它们来识别和剖析离子信号成分，从而帮助破解生物电密码。随着生物物理因素被纳入我们对细胞相互作用的系统级理解，将生物电事件作为指导性信号进行解剖，使细胞行为协调成大规模连贯的模式程序，将丰富生物学各个领域正在进行的工作。
Alongside the well-known chemical modes of cell-cell communication, we find an important and powerful system of bioelectrical signaling: changes in the resting voltage potential (Vmem) of the plasma membrane driven by ion channels, pumps and gap junctions. Slow Vmem changes in all cells serve as a highly conserved, information-bearing pathway that regulates cell proliferation, migration and differentiation. In embryonic and regenerative pattern formation and in the disorganization of neoplasia, bioelectrical cues serve as mediators of large-scale anatomical polarity, organ identity and positional information. Recent developments have resulted in tools that enable a high-resolution analysis of these biophysical signals and their linkage with upstream and downstream canonical genetic pathways. Here, we provide an overview for the study of bioelectric signaling, focusing on state-of-the-art approaches that use molecular physiology and developmental genetics to probe the roles of bioelectric events functionally. We highlight the logic, strategies and well-developed technologies that any group of researchers can employ to identify and dissect ionic signaling components in their own work and thus to help crack the bioelectric code. The dissection of bioelectric events as instructive signals enabling the orchestration of cell behaviors into large-scale coherent patterning programs will enrich on-going work in diverse areas of biology, as biophysical factors become incorporated into our systems-level understanding of cell interactions.
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影响因子：
12.4
作者：
Bashor CJ;Horwitz AA;Peisajovich SG;Lim WA
通讯作者：
Lim WA
影响因子：
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作者：
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通讯作者：
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作者：
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