Epithelial cell secretions from the human female reproductive tract inhibit sexually transmitted pathogens and Candida albicans but not Lactobacillus.
Epithelial cell secretions from the human female reproductive tract inhibit sexually transmitted pathogens and Candida albicans but not Lactobacillus.
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女性生殖道 (FRT) 上皮细胞可防止潜在的病原体和性传播感染。本研究的目的是确定上 FRT 的上皮细胞是否分泌抗菌素来抑制威胁女性健康的生殖道病原体。将输卵管、子宫、宫颈和宫颈外上皮细胞原代培养物的顶端分泌物与淋病奈瑟菌、白色念珠菌（酵母和菌丝形式）、HIV-1 和卷曲乳杆菌一起孵育，然后测试其生长和/或感染靶细胞的能力。上部 FRT 的上皮细胞分泌物可抑制淋病奈瑟菌和两种形式的念珠菌，并减少靶细胞的 HIV-1 (R5) 感染。相比之下，没有一个对卷曲乳杆菌具有抑制作用。子宫分泌物中的细胞因子和趋化因子分析揭示了几种可以解释病原体抑制的分子。这些发现为上皮细胞在保护 FRT 免受感染方面的关键作用提供了明确的证据，且不排除卷曲乳杆菌的有益存在，而卷曲乳杆菌是人类正常阴道微生物群的一部分。
Female reproductive tract (FRT) epithelial cells protect against potential pathogens and sexually transmitted infections. The purpose of this study was to determine if epithelial cells from the upper FRT secrete antimicrobials that inhibit reproductive tract pathogens which threaten women's health. Apical secretions from primary cultures of Fallopian tube, uterine, cervical and ectocervical epithelial cells were incubated with Neisseria gonorrhoeae, Candida albicans (yeast and hyphal forms), HIV-1, and Lactobacillus crispatus, prior to being tested for their ability to grow and/or infect target cells. Epithelial cell secretions from the upper FRT inhibit N. gonorrhoeae and both forms of Candida, as well as reduce HIV-1 (R5) infection of target cells. In contrast, none had an inhibitory effect on L. crispatus. Cytokines and chemokines analysis in uterine secretions revealed several molecules that could account for pathogen inhibition. These findings provide definitive evidence for the critical role of epithelial cells in protecting the FRT from infections, without comprising the beneficial presence of L. crispatus, which is part of the normal vaginal microflora of humans.
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