Proteome Analysis of Watery Saliva Secreted by Green Rice Leafhopper, Nephotettix cincticeps.
Proteome Analysis of Watery Saliva Secreted by Green Rice Leafhopper, Nephotettix cincticeps.
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DOI：
10.1371/journal.pone.0123671
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Matsumoto Y
中科院分区：
文献类型：
--
作者：
Hattori M;Komatsu S;Noda H;Matsumoto Y
绿色稻叶蝉是一种维管束取食者，在取食过程中分泌水状和凝胶状唾液。为了了解唾液在成功和安全地取食寄主植物中的潜在功能，我们分析了中华白蛉水唾液中蛋白质成分的复杂性。五头蛇唾液蛋白质是从成年叶蝉通过拉伸的石蜡膜膜喂养的蔗糖饮食中收集的。在还原和非还原条件下使用SDS-PAGE分离蛋白浓缩物。通过气相蛋白质测序仪鉴定了6个蛋白质，并参考表达序列标签（EST）数据库，使用LC-MS/MS分析鉴定了2个蛋白质。通过RACE-PCR和简并PCR获得了编码这些主要蛋白的全长cDNA。此外，参照最新的RNA测序数据，我们进行了无凝胶蛋白质组分析，以涵盖广泛的唾液蛋白质。cincticeps，产生了63个额外的蛋白质种类。在从水样唾液中鉴定出的71种新蛋白质中，约60%是酶或其他功能蛋白质，包括GH 5纤维素酶、转铁蛋白、碳酸酐酶、氨肽酶、regucalcin和载脂蛋白。其余的蛋白质似乎是独特的和物种特异性的。这是第一个研究，以确定和表征的蛋白质在水性唾液的Auchelphyryncha物种，特别是鞘生产，血管的食饵动物。
The green rice leafhopper, Nephotettix cincticeps, is a vascular bundle feeder that discharges watery and gelling saliva during the feeding process. To understand the potential functions of saliva for successful and safe feeding on host plants, we analyzed the complexity of proteinaceous components in the watery saliva of N. cincticeps. Salivary proteins were collected from a sucrose diet that adult leafhoppers had fed on through a membrane of stretched parafilm. Protein concentrates were separated using SDS-PAGE under reducing and non-reducing conditions. Six proteins were identified by a gas-phase protein sequencer and two proteins were identified using LC-MS/MS analysis with reference to expressed sequence tag (EST) databases of this species. Full -length cDNAs encoding these major proteins were obtained by rapid amplification of cDNA ends-PCR (RACE-PCR) and degenerate PCR. Furthermore, gel-free proteome analysis that was performed to cover the broad range of salivary proteins with reference to the latest RNA-sequencing data from the salivary gland of N. cincticeps, yielded 63 additional protein species. Out of 71 novel proteins identified from the watery saliva, about 60 % of those were enzymes or other functional proteins, including GH5 cellulase, transferrin, carbonic anhydrases, aminopeptidase, regucalcin, and apolipoprotein. The remaining proteins appeared to be unique and species- specific. This is the first study to identify and characterize the proteins in watery saliva of Auchenorrhyncha species, especially sheath-producing, vascular bundle-feeders.
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DOI：
10.1016/j.cbpb.2008.02.009
发表时间：
2008-06-01
影响因子：
2.2
作者：
Kim, Bo Yeon;Lee, Kwang Sik;Jin, Byung Rae
通讯作者：
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影响因子：
1.9
作者：
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影响因子：
2.2
作者：
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DOI：
10.1073/pnas.90.4.1320
发表时间：
1993-02-15
影响因子：
11.1
作者：
JAMROZ, RC;GASDASKA, JR;LAW, JH
通讯作者：
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影响因子：
7.3
作者：
De Vos, Martin;Jander, Georg
通讯作者：
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