Manipulation of mammalian cells using a single-fiber optical microbeam.

Manipulation of mammalian cells using a single-fiber optical microbeam.
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DOI:
10.1117/1.2983663
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发表时间:
2008-09
影响因子:
3.5
通讯作者:
Berns MW
Berns MW
中科院分区:
医学3区
文献类型:
--
作者:
Mohanty SK;Mohanty KS;Berns MW

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显微镜物镜的短工作距离严重限制了光学微操作技术在更大深度的应用。我们展示了第一次使用光纤镊子对神经元生长锥和神经元拉伸的控制指导。此外,通过锁模,光纤镊子束被转换为光纤剪刀,使得能够解剖神经元过程,从而允许研究神经元对局部损伤的后续反应。在高平均功率下,完成了三维捕获的细胞的裂解。
The short working distance of microscope objectives has severely restricted the application of optical micromanipulation techniques at larger depths. We show the first use of fiber-optic tweezers toward controlled guidance of neuronal growth cones and stretching of neurons. Further, by mode locking, the fiber-optic tweezers beam was converted to fiber-optic scissors, enabling dissection of neuronal processes and thus allowing study of the subsequent response of neurons to localized injury. At high average powers, lysis of a three-dimensionally trapped cell was accomplished.
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