KLF13 promotes porcine adipocyte differentiation through PPARγ activation.
KLF13 promotes porcine adipocyte differentiation through PPARγ activation.
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DOI：
10.1186/s13578-015-0016-z
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发表时间：
2015
影响因子：
7.5
通讯作者：
Jiang S
中科院分区：
文献类型：
--
作者：
Jiang S;Wei H;Song T;Yang Y;Zhang F;Zhou Y;Peng J;Jiang S
脂肪生成是由一个复杂的网络转录因子在不同阶段的分化作用。Kruppel样因子（KLFs）是一类锌指转录因子家族，在哺乳动物细胞分化和发育过程中发挥着重要作用。在本研究中，我们发现KLF 13是调节猪脂肪细胞分化的关键调节因子。在猪脂肪细胞分化的早期，KLF 13的表达显著上调，随后是PPARγ的表达。猪脂肪细胞的分化显着减弱，通过添加针对KLF 13的siRNA，而KLF 13的过表达导致增强猪脂肪细胞的分化。通过启动子缺失和突变分析，我们在猪PPARγ近端启动子的−593/-577区域内鉴定了一个KLF 13结合位点，表明KLF 13直接与猪PPARγ启动子相互作用。然而，通过siRNA抑制KLF 13并不损害小鼠脂肪细胞分化。此外，在3个T3-L1细胞中敲低和/或过表达KLF 13均不影响PPARγ2的表达。总的来说，我们的研究结果表明，KLF 13作为一个关键的前脂肪形成转录因子存在，通过反式激活PPARγ表达在猪脂肪细胞分化，而没有这样的效果在小鼠脂肪细胞分化检测。本文的在线版本（doi：10.1186/s13578-015-0016-z）包含补充材料，可供授权用户使用。
Adipogenesis is tightly controlled by a complex network of transcription factors acting at different stages of differentiation. Kruppel-like factors (KLFs) as a family of zinc-finger transcription factors play diverse roles during cell differentiation and development in mammals. In the present study, we showed that KLF13 acts as a key regulator regulating porcine adipocyte differentiation. The expression of KLF13 was markedly up-regulated during the early stage of porcine adipocyte differentiation, which was followed by expression of PPARγ. Porcine adipocyte differentiation was significantly attenuated by the addition of siRNA against KLF13, whereas overexpression of KLF13 resulted in enhanced porcine adipocyte differentiation. Using promoter deletion and mutation analysis, we identified a KLF13-binding site within −593/-577 region of the porcine PPARγ proximal promoter, indicating that KLF13 directly interacts with porcine PPARγ promoter. However, inhibition of KLF13 by siRNA did not impair mouse adipocyte differentiation. In addition, knockdown and/or overexpression of KLF13 in 3 T3-L1 cells all did not influence expression of PPARγ2. Collectively, our results suggest that KLF13 exist as a key pro-adipogenic transcription factor through transactivating PPARγ expression in porcine adipocyte differentiation, whereas no such effect was detected in mouse adipocyte differentiation. The online version of this article (doi:10.1186/s13578-015-0016-z) contains supplementary material, which is available to authorized users.
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