3D bioprinting of a corneal stroma equivalent.
3D bioprinting of a corneal stroma equivalent.
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DOI：
10.1016/j.exer.2018.05.010
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发表时间：
2018-08
影响因子：
3.4
通讯作者：
Connon CJ
中科院分区：
文献类型：
--
作者：
Isaacson A;Swioklo S;Connon CJ
角膜移植是治疗严重角膜功能丧失的主要方法之一。由于其局限性，组织工程师已经做出了一致的努力来生产功能性的合成角膜假体作为替代手段。然而，这些疗法尚未成功地转化为临床。3D生物打印是一种新兴技术，可用于制造临床应用的生物组织。我们将其应用于角膜组织工程领域，以便使用现有的3D数字人角膜模型和合适的支撑结构来制造类似于天然人角膜基质结构的角膜结构。这些是从内部胶原基生物墨水中3D打印出来的，其中包含封装的角膜角膜细胞。角膜细胞在打印后第1天（>90%）和第7天（83%）均表现出高细胞活力。我们将3D生物打印确定为一种可行的方法，通过这种方法可以设计人工角膜结构。
Corneal transplantation constitutes one of the leading treatments for severe cases of loss of corneal function. Due to its limitations, a concerted effort has been made by tissue engineers to produce functional, synthetic corneal prostheses as an alternative recourse. However, successful translation of these therapies into the clinic has not yet been accomplished. 3D bioprinting is an emerging technology that can be harnessed for the fabrication of biological tissue for clinical applications. We applied this to the area of corneal tissue engineering in order to fabricate corneal structures that resembled the structure of the native human corneal stroma using an existing 3D digital human corneal model and a suitable support structure. These were 3D bioprinted from an in-house collagen-based bio-ink containing encapsulated corneal keratocytes. Keratocytes exhibited high cell viability both at day 1 post-printing (>90%) and at day 7 (83%). We established 3D bio-printing to be a feasible method by which artificial corneal structures can be engineered.
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影响因子：
17.8
作者：
Ruberti, Jeffrey W.;Zieske, James D.
通讯作者：
Zieske, James D.
影响因子：
13.6
作者：
Hinton TJ;Jallerat Q;Palchesko RN;Park JH;Grodzicki MS;Shue HJ;Ramadan MH;Hudson AR;Feinberg AW
通讯作者：
Feinberg AW
影响因子：
9
作者：
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通讯作者：
Forgacs G
影响因子：
4.4
作者：
Gouveia, Ricardo Martins;Connon, Che John
通讯作者：
Connon, Che John
影响因子：
14
作者：
Klock, G;Pfeffermann, A;Zimmermann, U
通讯作者：
Zimmermann, U