Tissue engineering by self-assembly and bio-printing of living cells.
Tissue engineering by self-assembly and bio-printing of living cells.
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DOI：
10.1088/1758-5082/2/2/022001
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发表时间：
2010-06
期刊：
影响因子：
9
通讯作者：
Forgacs G
中科院分区：
文献类型：
--
作者：
Jakab K;Norotte C;Marga F;Murphy K;Vunjak-Novakovic G;Forgacs G
生物制造具有所需的拓扑结构和功能需要物理、生命、医学和工程科学从业者的跨学科努力。世界各地的许多实验室都在进行这样的努力。目前正在寻求多种方法，例如使用天然或人造支架、脱细胞身体细胞外基质和最近的生物打印。为了在这一努力中取得成功，为与生物体中的细胞相似的细胞提供体外微环境线索至关重要。因此，填充了分化细胞或干细胞的支架正在被制造出来，这些细胞的复杂性和复杂性越来越高。然而，无论支架多么复杂，它们都可能因其降解而引起问题，引发免疫原性反应和其他事先无法预见的并发症。人们还认识到，归根结底，最好的方法是依靠细胞和组织的自组装和自组织特性以及生物体本身固有的再生能力，而不是简单地在体外准备组织和器官结构，然后将其植入。在这里，我们简要回顾了制造三维生物结构的不同策略，特别是生物打印。我们详细介绍了一种完全生物的、无支架的、基于印刷的工程方法，该方法使用自组装的多细胞单元作为生物墨水颗粒，并采用早期发育形态发生原理，如细胞分选和组织融合。
Biofabrication of living structures with desired topology and functionality requires the interdisciplinary effort of practitioners of the physical, life, medical and engineering sciences. Such efforts are being undertaken in many laboratories around the world. Numerous approaches are being pursued, such as those based on the use of natural or artificial scaffolds, decellularized cadaveric extracellular matrices and lately bioprinting. To be successful in this endeavor it is crucial to provide in vitro micro-environmental clues for the cells resembling those in the organism. Therefore scaffolds populated with differentiated cells or stem cells of increasing complexity and sophistication are being fabricated. However, scaffolds, no matter how sophisticated they are, can cause problems stemming from their degradation, eliciting immunogenic reactions and other a priori unforeseen complications. It is also being realized that ultimately the best approach is to rely on the self-assembly and self-organizing properties of cells and tissues and the innate regenerative capability of the organism itself, not just simply prepare tissue and organ structures in vitro followed by their implantation. Here we briefly review the different strategies for the fabrication of three-dimensional biological structures, in particular bioprinting. We detail a fully biological, scaffoldless, print-based engineering approach that uses self-assembling multicellular units as bioink particles and employs early developmental morphogenetic principles, such as cell sorting and tissue fusion.
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影响因子：
4
作者：
Freytes, Donald O.;Wan, Leo Q.;Vunjak-Novakovic, Gordana
通讯作者：
Vunjak-Novakovic, Gordana
影响因子：
4.7
作者：
Boland, Thomas;Xu, Tao;Cui, Xiaofeng
通讯作者：
Cui, Xiaofeng
影响因子：
4.6
作者：
Campbell, Phil G.;Weiss, Lee E.
通讯作者：
Weiss, Lee E.
DOI：
10.1126/science.1171643
发表时间：
2009-06-26
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Discher DE;Mooney DJ;Zandstra PW
通讯作者：
Zandstra PW
DOI：
10.1115/1.1449491
发表时间：
2002-04-01
影响因子：
1.7
作者：
Brodland, GW
通讯作者：
Brodland, GW