Hemagglutinin of influenza virus partitions into the nonraft domain of model membranes.
Hemagglutinin of influenza virus partitions into the nonraft domain of model membranes.
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流感病毒的血凝素分配到模型膜的非筏域中。
DOI：
10.1016/j.bpj.2010.04.027
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发表时间：
2010
影响因子：
3.4
通讯作者：
A. Herrmann
中科院分区：
文献类型：
--
作者：
Jörg Nikolaus;Silvia Scolari;E. Bayraktarov;N. Jungnick;S. Engel;Anna Pia Plazzo;M. Stöckl;R. Volkmer;M. Veit;A. Herrmann
流感病毒的HA是跨膜蛋白的范例，其被认为与富含胆固醇、鞘糖脂和饱和磷脂的质膜的膜筏、液体有序样纳米结构域相关。由于其在细胞中的亚微米尺寸，筏不能直接可视化，迄今为止通过间接方法分析HA的筏缔合。在这项研究中，我们已经使用GUV和GPMV，显示液体无序和液体有序域，直接可视化分区的HA荧光显微镜。我们表明，HA是专门的（GUV）或主要（GPMV）存在于液体无序域，无论是否真正的HA或域包含其筏靶向信号重建成模型膜。HA到LD域的优先分区和GUV和GPMV中的LO分区之间的差异进行了讨论相对于模型系统和活细胞膜中的脂质包装的差异，这表明生物膜中的脂质结构域的物理性质受到蛋白质-脂质相互作用的严格调控。
The HA of influenza virus is a paradigm for a transmembrane protein thought to be associated with membrane-rafts, liquid-ordered like nanodomains of the plasma membrane enriched in cholesterol, glycosphingolipids, and saturated phospholipids. Due to their submicron size in cells, rafts can not be visualized directly and raft-association of HA was hitherto analyzed by indirect methods. In this study, we have used GUVs and GPMVs, showing liquid disordered and liquid ordered domains, to directly visualize partition of HA by fluorescence microscopy. We show that HA is exclusively (GUVs) or predominantly (GPMVs) present in the liquid disordered domain, regardless of whether authentic HA or domains containing its raft targeting signals were reconstituted into model membranes. The preferential partition of HA into ld domains and the difference between lo partition in GUV and GPMV are discussed with respect to differences in packaging of lipids in membranes of model systems and living cells suggesting that physical properties of lipid domains in biological membranes are tightly regulated by protein-lipid interactions.
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DOI：
--
发表时间：
2003
影响因子：
11.1
作者：
Mamoru Takeda
通讯作者：
Mamoru Takeda
影响因子：
3.7
作者：
Leser, GP;Lamb, RA
通讯作者：
Lamb, RA
影响因子：
14.8
作者：
Polozov, Ivan V.;Bezrukov, Ludmila;Zimmerberg, Joshua
通讯作者：
Zimmerberg, Joshua
影响因子：
3.4
作者：
Honerkamp-Smith, Aurelia R.;Veatch, Sarah L.;Keller, Sarah L.
通讯作者：
Keller, Sarah L.
流感血凝素跨膜结构域的二级结构、方向、寡聚化和脂质相互作用。
影响因子：
2.9
作者：
Tatulian,SA;Tamm,LK
通讯作者：
Tamm,LK