Drosophila social clustering is disrupted by anesthetics and in narrow abdomen ion channel mutants.
Drosophila social clustering is disrupted by anesthetics and in narrow abdomen ion channel mutants.
复制标题
果蝇社交聚类被麻醉和腹部离子通道突变体所破坏。
DOI：
10.1111/gbb.12025
复制
发表时间：
2013-04
期刊：
影响因子：
--
通讯作者：
Nash HA
中科院分区：
文献类型：
--
作者：
Burg ED;Langan ST;Nash HA
许多物种的成员往往聚集在一起，这种行为策略被称为局部增强。局部增强的选择性优势包括有效利用资源和防御捕食者。虽然之前的研究已经研究了果蝇的许多类型的社会行为，但很少有专门研究局部增强的。最近在果蝇中使用一种名为社会空间指数的测量方法描述了资源独立的局部增强，尽管神经机制仍不清楚。在这里，我们分析了果蝇在不依赖资源的情况下局部增强的情况，这使我们能够阐明其神经机制。我们已经研究了普通挥发性麻醉剂的效果，这种化合物损害了对社会暗示做出反应所需的类型的高级功能。我们将广州-S果蝇暴露在非固定浓度的氟烷中，发现与在空气中测试的果蝇相比，果蝇的社会空间指数显著降低。窄腹(NA)突变体在许多行为测试中对麻醉剂的反应发生了变化，也显著降低了社会空间指数，这种影响可以通过用NA-GAL4驱动UAS-NA救援结构来恢复NA的表达而完全逆转。我们发现，NA在胆碱能神经元中的表达可以完全挽救行为缺陷，而NA在谷氨酸能神经元中的表达只能部分恢复行为缺陷。我们的结果还表明，NA在蘑菇体中的表达也起到了作用，因为抑制NA-GAL4救援苍蝇蘑菇体中NA的表达会降低社会空间指数。我们的数据表明，资源无关的局部增强是一种简单的行为策略，需要复杂的神经处理。
Members of many species tend to congregate, a behavioral strategy known as local enhancement. Selective advantages of local enhancement range from efficient use of resources to defense from predators. While previous studies have examined many types of social behavior in fruit flies, few have specifically investigated local enhancement. Resource-independent local enhancement has recently been described in the fruit fly using a measure called social space index, although the neural mechanisms remain unknown. Here we analyze resource-independent local enhancement of Drosophila under conditions that allow us to elucidate its neural mechanisms. We have investigated the effects of general volatile anesthetics, compounds that compromise higher order functioning of the type typically required for responding to social cues. We exposed Canton-S flies to non-immobilizing concentrations of halothane and found that flies had a significantly decreased social space index compared to flies tested in air. Narrow abdomen (na) mutants, which display altered responses to anesthetics in numerous behavioral assays, also have a significantly reduced social space index, an effect that was fully reversed by restoring expression of na by driving a UAS-NA rescue construct with NA-GAL4. We found that na expression in cholinergic neurons fully rescued the behavioral defect, whereas expression of na in glutamatergic neurons did so only partially. Our results also suggest a role for na expression in the mushroom bodies, since suppressing na expression in the mushroom bodies of NA-GAL4 rescue flies diminishes social space index. Our data indicate that resource-independent local enhancement, a simple behavioral strategy, requires complex neural processing.
登录
查看更多内容
影响因子：
2
作者：
Diegelmann, S;Zars, M;Zars, T
通讯作者：
Zars, T
影响因子：
9.2
作者：
Humphrey, John A.;Hamming, Kevin S.;Nash, Howard A.
通讯作者：
Nash, Howard A.
影响因子：
9.2
作者：
Battesti, Marine;Moreno, Celine;Mery, Frederic
通讯作者：
Mery, Frederic
影响因子：
2.3
作者：
BARTELT, RJ;SCHANER, AM;JACKSON, LL
通讯作者：
JACKSON, LL
影响因子：
2
作者：
Mehren, Jennifer E.;Griffith, Leslie C.
通讯作者：
Griffith, Leslie C.