Discordant interactions between YAP1 and polycomb group protein SCML2 determine cell fate.
Discordant interactions between YAP1 and polycomb group protein SCML2 determine cell fate.
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DOI：
10.1016/j.isci.2023.107964
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发表时间：
2023-10-20
期刊：
影响因子：
5.8
通讯作者：
Cinar B
中科院分区：
文献类型：
--
作者：
Boston AM;Dwead AM;Al-Mathkour MM;Khazaw K;Zou J;Zhang Q;Wang G;Cinar B
The Polycomb group protein SCML2 and the transcriptional cofactor YAP1 regulate diverse cellular biology, including stem cell maintenance, developmental processes, and gene regulation in mammals and flies. However, their molecular and functional interactions are unknown. Here, we show that SCML2 interacts with YAP1, as revealed by immunological assays and mass spectroscopy. We have demonstrated that the steroid hormone androgen regulates the interaction of SCML2 with YAP1 in human tumor cell models. Our proximity ligation assay and GST pulldown showed that SCML2 and YAP1 physically interacted with each other. Silencing SCML2 by RNAi changed the growth behaviors of cells in response to androgen signaling. Mechanistically, this phenomenon is attributed to the interplay between distinct chromatin modifications and transcriptional programs, likely coordinated by the opposing SCML2 and YAP1 activity. These findings suggest that YAP1 and SCML2 cooperate to regulate cell growth, cell survival, and tumor biology downstream of steroid hormones. Polycomb repressive protein SCML2 biochemically and functionally interacts with YAP1 SCML2 depletion alters cell growth in response to streoid hormone androgen signaling SCML2 and YAP1 inversely modulate H3K27me3 and H2AK119Ub chromatin modifications Cooperative SCML2 and YAP1 signaling regulates cell growth and cell survival Molecular biology; Cell biology; Omics; Proteomics
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影响因子：
64.8
作者：
Chang L;Azzolin L;Di Biagio D;Zanconato F;Battilana G;Lucon Xiccato R;Aragona M;Giulitti S;Panciera T;Gandin A;Sigismondo G;Krijgsveld J;Fassan M;Brusatin G;Cordenonsi M;Piccolo S
通讯作者：
Piccolo S
影响因子：
7.3
作者：
Gao J;Aksoy BA;Dogrusoz U;Dresdner G;Gross B;Sumer SO;Sun Y;Jacobsen A;Sinha R;Larsson E;Cerami E;Sander C;Schultz N
通讯作者：
Schultz N
影响因子：
16.6
作者：
Kuser-Abali G;Alptekin A;Lewis M;Garraway IP;Cinar B
通讯作者：
Cinar B
影响因子：
4.5
作者：
Adams SR;Maezawa S;Alavattam KG;Abe H;Sakashita A;Shroder M;Broering TJ;Sroga Rios J;Thomas MA;Lin X;Price CM;Barski A;Andreassen PR;Namekawa SH
通讯作者：
Namekawa SH
DOI：
10.1038/s41580-021-00398-y
发表时间：
2021-12
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
Blackledge NP;Klose RJ
通讯作者：
Klose RJ