Live visualisation of electrolytes during mouse embryonic development using electrolyte indicators.
Live visualisation of electrolytes during mouse embryonic development using electrolyte indicators.
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DOI：
10.1371/journal.pone.0246337
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Terada Y
中科院分区：
文献类型：
--
作者：
Fujishima A;Takahashi K;Goto M;Hirakawa T;Iwasawa T;Togashi K;Maeda E;Shirasawa H;Miura H;Sato W;Kumazawa Y;Terada Y
研究表明，一些电解质，包括Na+和K+，在胚胎发育中发挥着重要作用。然而，这些研究通过使用抑制剂或基因敲除小鼠来评估这些电解质，但没有提及胚胎发生过程中细胞内电解质浓度的变化。在本研究中，我们使用电解质指示剂 CoroNa Green AM 和 ION Potassium Green-2 AM 分别直接可视化小鼠胚胎每个胚胎发育阶段的细胞内 Na+ 和 K+ 浓度。我们直接观察桑椹胚、囊胚和孵化阶段的细胞内电解质浓度。我们的结果揭示了细胞内电解质浓度的动态变化；我们发现囊胚腔形成过程中细胞内Na+浓度降低，而K+浓度升高。对哇巴因（Na+/K+ ATP酶抑制剂）反应强度的变化程度被认为与每个发育阶段的Na+/K+ ATP酶活性程度相对应。此外，在囊胚阶段后，与囊胚腔直接接触的滋养外胚层细胞比与内细胞团直接接触的滋养外胚层细胞显示出更高的 K+ 浓度，表明 Na+/K+ ATPase 活性根据滋养外胚层中的位置而有所不同。这是第一项在小鼠胚胎中使用 CoroNa Green AM 和 ION Potassium Green-2 AM 并可视化胚胎发育过程中电解质的研究。本研究中观察到的电解质浓度变化与之前报道的Na+/K+ ATP酶活性一致，并且可以对胚胎细胞中更详细的电解质行为进行成像。该方法可用于提高对细胞生理学的理解，并对未来的胚胎发育研究有用。
Studies have shown that some electrolytes, including Na+ and K+, play important roles in embryonic development. However, these studies evaluated these electrolytes by using inhibitors or knockout mice, with no mention on the changes in the intracellular electrolyte concentrations during embryogenesis. In this study, we used the electrolyte indicators CoroNa Green AM and ION Potassium Green-2 AM to directly visualise intracellular concentrations of Na+ and K+, respectively, at each embryonic developmental stage in mouse embryos. We directly observed intracellular electrolyte concentrations at the morula, blastocyst, and hatching stages. Our results revealed dynamic changes in intracellular electrolyte concentrations; we found that the intracellular Na+ concentration decreased, while K+ concentration increased during blastocoel formation. The degree of change in intensity in response to ouabain, an inhibitor of Na+/K+ ATPase, was considered to correspond to the degree of Na+/K+ ATPase activity at each developmental stage. Additionally, after the blastocyst stage, trophectoderm cells in direct contact with the blastocoel showed higher K+ concentrations than in direct contact with inner cell mass, indicating that Na+/K+ ATPase activity differs depending on the location in the trophectoderm. This is the first study to use CoroNa Green AM and ION Potassium Green-2 AM in mouse embryos and visualise electrolytes during embryonic development. The changes in electrolyte concentration observed in this study were consistent with the activity of Na+/K+ ATPase reported previously, and it was possible to image more detailed electrolyte behaviour in embryo cells. This method can be used to improve the understanding of cell physiology and is useful for future embryonic development studies.
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影响因子：
2.7
作者：
Boyle, Scott;Misfeldt, Andrew;de Caestecker, Mark
通讯作者：
de Caestecker, Mark
影响因子：
--
作者：
Sekar P;Huang DY;Chang SF;Lin WW
通讯作者：
Lin WW
DOI：
10.1007/s00249-016-1173-7
发表时间：
2016-10
影响因子：
2
作者：
Iamshanova, Oksana;Mariot, Pascal;Lehen'kyi, V'yacheslav;Prevarskaya, Natalia
通讯作者：
Prevarskaya, Natalia
影响因子：
3
作者：
Meier, Silke D.;Kovalchuk, Yury;Rose, Christine R.
通讯作者：
Rose, Christine R.
DOI：
10.1002/jez.a.90
发表时间：
2004-09-01
影响因子：
2.8
作者：
Kawagishi, R;Tahara, M;Murata, Y
通讯作者：
Murata, Y