Rapid visualization of Acanthamoeba using fluorescein-conjugated lectins.
Rapid visualization of Acanthamoeba using fluorescein-conjugated lectins.
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使用荧光素结合的凝集素快速观察棘阿米巴。
DOI：
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
B. Andersen
中科院分区：
文献类型：
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作者：
J. Robin;R. Chan;B. Andersen
我们研究了荧光素结合的凝集素 (FCL) 对棘阿米巴物种快速可视化的功效。在补充有大肠杆菌的非营养琼脂平板上建立卡斯氏棘阿米巴、库伯氏棘阿米巴和多食棘阿米巴的培养物。初始传代培养后四到五天建立最大滋养体种群；三到六天后通常会发现成熟的包囊。在不同的时间点，收获滋养体和/或包囊并悬浮在佩吉盐水中。按照先前描述的方案，将悬浮液样品放置在载玻片上并与 14 个 FCL 组中的一个一起孵育。在孵育期结束时，使用落射荧光显微镜检查载玻片。伴刀豆球蛋白 A 包囊和滋养体均染色明亮。小麦胚芽凝集素使包囊染色明亮，但滋养体仅染色最低限度。这些结果表明 FCL 可能有助于快速观察角膜感染中的棘阿米巴微生物。
We investigated the efficacy of fluorescein-conjugated lectins (FCLs) for the rapid visualization of Acanthamoeba species. Cultures of Acanthamoeba castellani, Acanthamoeba culbertsoni, and Acanthamoeba polyphaga were established on nonnutrient agar plates supplemented with Escherichia coli. Maximal trophozoite populations were established four to five days after initial subculturing; mature cysts were routinely noted three to six days later. At various time points, trophozoites and/or cysts were harvested and suspended in Page's saline. Following a previously described protocol, samples of the suspensions were placed on glass slides and incubated with one of a panel of 14 FCLs. At the end of the incubation period, the slides were examined using an epifluorescence microscope. Concanavalin A brightly stained both cysts and trophozoites. Wheat germ agglutinin brightly stained cysts but only minimally stained trophozoites. These results suggest that FCLs may be useful for rapid visualization of Acanthamoeba organisms in corneal infections.
使用荧光素结合的凝集素快速可视化三种常见真菌。
影响因子：
4.4
作者：
Robin,JB;Arffa,RC;Avni,I;Rao,NA
通讯作者：
Rao,NA
用于识别真菌的卡尔科弗卢和墨水-氢氧化钾制剂。
影响因子：
4.2
作者：
Arffa,RC;Avni,I;Ishibashi,Y;Robin,J;Kaufman,HE
通讯作者：
Kaufman,HE
放射状角膜神经炎是棘阿米巴角膜炎的症状。
影响因子：
13.7
作者：
Moore,MB;McCulley,JP;Kaufman,HE;Robin,JB
通讯作者：
Robin,JB