The golgin GMAP-210 is required for efficient membrane trafficking in the early secretory pathway.
The golgin GMAP-210 is required for efficient membrane trafficking in the early secretory pathway.
复制标题
DOI：
10.1242/jcs.166710
复制
发表时间：
2015-04-15
影响因子：
4
通讯作者：
Lowe M
中科院分区：
文献类型：
--
作者：
Roboti P;Sato K;Lowe M
高尔基体是一种卷曲螺旋蛋白，参与高尔基体复合体的膜束缚作用。高尔基体介导的束缚被认为是重要的囊泡运输和高尔基体组织。然而，在何种程度上个别golgins有助于这些过程是不明确的，它已被提出，golgins在很大程度上是冗余的方式。以前对golgin GMAP-210（也称为TRIP 11）的研究，在罕见的骨骼疾病软骨发育不全型1A中发生突变，在其参与贩运方面产生了相互矛盾的结果。在这里，我们重新研究了GMAP-210的运输作用，发现它确实是分泌途径中有效运输所必需的。GMAP-210在顺行运输过程中作用于内质网（ER）-高尔基中间室（ERGIC）和高尔基复合体，并且也是逆行运输到ER所需的。使用共耗竭实验，我们还发现，GMAP-210的行为在一个部分冗余的方式与高尔基GM 130，以确保有效的顺行货物交付的顺式高尔基体。总之，我们的研究结果表明GMAP-210在ER-高尔基体界面的几个贩运步骤中的作用，其中一些与另一种高尔基体，即GM 130（也称为GOLGA 2）部分冗余。
Golgins are coiled-coil proteins that participate in membrane-tethering events at the Golgi complex. Golgin-mediated tethering is thought to be important for vesicular trafficking and Golgi organization. However, the degree to which individual golgins contribute to these processes is poorly defined, and it has been proposed that golgins act in a largely redundant manner. Previous studies on the golgin GMAP-210 (also known as TRIP11), which is mutated in the rare skeletal disorder achondrogenesis type 1A, have yielded conflicting results regarding its involvement in trafficking. Here, we re-investigated the trafficking role of GMAP-210, and found that it is indeed required for efficient trafficking in the secretory pathway. GMAP-210 acts at both the endoplasmic reticulum (ER)-to-Golgi intermediate compartment (ERGIC) and Golgi complex during anterograde trafficking, and is also required for retrograde trafficking to the ER. Using co-depletion experiments, we also found that GMAP-210 acts in a partially redundant manner with the golgin GM130 to ensure efficient anterograde cargo delivery to the cis-Golgi. In summary, our results indicate a role for GMAP-210 in several trafficking steps at the ER–Golgi interface, some of which are partially redundant with another golgin, namely GM130 (also known as GOLGA2).
登录
查看更多内容
影响因子：
3.7
作者：
Koreishi M;Gniadek TJ;Yu S;Masuda J;Honjo Y;Satoh A
通讯作者：
Satoh A
影响因子：
3.3
作者：
Marra, Pierfrancesco;Salvatore, Lorena;De Matteis, Maria Antonietta
通讯作者：
De Matteis, Maria Antonietta
影响因子：
20.3
作者：
Bremond, Aurore;Meynet, Ophelie;Bernard, Ghislaine
通讯作者：
Bernard, Ghislaine
DOI：
10.1073/pnas.0800137105
发表时间：
2008-03-04
影响因子：
11.1
作者：
Lieu, Zi Zhao;Lock, John G.;Gleeson, Paul A.
通讯作者：
Gleeson, Paul A.
影响因子：
4
作者：
Broekhuis, Joost R.;Rademakers, Suzanne;Jansen, Gert
通讯作者：
Jansen, Gert