The golgin tether giantin regulates the secretory pathway by controlling stack organization within Golgi apparatus.
The golgin tether giantin regulates the secretory pathway by controlling stack organization within Golgi apparatus.
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DOI：
10.1371/journal.pone.0059821
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Satoh A
中科院分区：
文献类型：
--
作者：
Koreishi M;Gniadek TJ;Yu S;Masuda J;Honjo Y;Satoh A
高尔基体是一种卷曲螺旋蛋白，在高尔基体结构和功能的调节中起着关键作用。Giantin是哺乳动物中最大的高尔基体蛋白，与p115、rab 1、GM 130和可溶性N-乙基马来酰亚胺敏感因子附着蛋白受体（SNARE）形成复合物，从而促进高尔基体周围的囊泡束缚和融合过程。用微管不稳定药物诺考达唑处理，将高尔基带转化为单独的高尔基堆。在这里，我们表明，siRNA介导的Giantin的消耗导致更分散的高尔基体堆栈后，诺考达唑治疗比对照治疗，而不改变平均脑池长度。此外，Giantin的耗尽引起与改变的细胞表面蛋白糖基化相关的货物转运的增加。已知果蝇S2细胞具有分散的高尔基体堆叠，并且没有Giantin同系物。在S2细胞中外源表达哺乳动物巨噬细胞蛋白cDNA导致聚集的高尔基体堆叠，类似于哺乳动物细胞中的高尔基带。这些结果表明，高尔基带的空间组织介导的giantin，这也发挥了作用，在货物运输和糖修饰。
Golgins are coiled-coil proteins that play a key role in the regulation of Golgi architecture and function. Giantin, the largest golgin in mammals, forms a complex with p115, rab1, GM130, and soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), thereby facilitating vesicle tethering and fusion processes around the Golgi apparatus. Treatment with the microtubule destabilizing drug nocodazole transforms the Golgi ribbon into individual Golgi stacks. Here we show that siRNA-mediated depletion of giantin resulted in more dispersed Golgi stacks after nocodazole treatment than by control treatment, without changing the average cisternal length. Furthermore, depletion of giantin caused an increase in cargo transport that was associated with altered cell surface protein glycosylation. Drosophila S2 cells are known to have dispersed Golgi stacks and no giantin homolog. The exogenous expression of mammalian giantin cDNA in S2 cells resulted in clustered Golgi stacks, similar to the Golgi ribbon in mammalian cells. These results suggest that the spatial organization of the Golgi ribbon is mediated by giantin, which also plays a role in cargo transport and sugar modifications.
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影响因子：
4.5
作者：
Satoh, A;Wang, Y;Warren, G
通讯作者：
Warren, G
影响因子：
4.5
作者：
Gniadek, Thomas J.;Warren, Graham
通讯作者：
Warren, Graham
DOI：
10.1083/jcb.131.6.1715
发表时间：
1995-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Nakamura N;Rabouille C;Watson R;Nilsson T;Hui N;Slusarewicz P;Kreis TE;Warren G
通讯作者：
Warren G
影响因子：
56.9
作者：
Malsam, J;Satoh, A;Warren, G
通讯作者：
Warren, G
影响因子：
64.8
作者：
Seemann, J;Jokitalo, E;Warren, G
通讯作者：
Warren, G