Antimicrobial, Antibiofilm, and Antioxidant Properties of Boletus edulis and Neoboletus luridiformis Against Multidrug-Resistant ESKAPE Pathogens.
Antimicrobial, Antibiofilm, and Antioxidant Properties of Boletus edulis and Neoboletus luridiformis Against Multidrug-Resistant ESKAPE Pathogens.
复制标题
DOI：
10.3389/fnut.2021.773346
复制
发表时间：
2021
影响因子：
5
通讯作者：
Saavedra MJ
中科院分区：
文献类型：
--
作者：
Garcia J;Rodrigues F;Castro F;Aires A;Marques G;Saavedra MJ
多重耐药ESKAPE病原体（屎肠球菌、金黄色葡萄球菌、肺炎克雷伯菌、鲍曼不动杆菌、铜绿假单胞菌和肠杆菌属）已成为全球最常见的皮肤和软组织感染原因，属于WHO优先病原体列表。成功的治疗仍然具有挑战性，需要评估新的和成功的抗生素。在这项研究中，蘑菇被认为是天然抗菌化合物的宝贵和独特的来源。因此，本研究旨在评价美味牛肝菌（B. edulis）和新牛肝菌Neoboletus luridiformis（N. luridiformis）的水提取物和甲醇提取物对来自临床伤口感染的ESKAPE分离物的作用。采用纸片扩散法和微量稀释法测定其抑菌活性。植物化学特性实现了总酚，邻二酚含量和抗氧化活性的分析，以及通过高效液相色谱-二极管阵列检测器（HPLC-DAD）。人包皮成纤维细胞-1（HFF-1）的细胞活力通过MTT测定进行。B的水和甲醇提取物。edulis和N.尽管具有不同的功效率，但luridiformis显示出抗多药耐药细菌的抗微生物和抗微生物膜特性。结果表明，有一个令人信服的酚类化合物的含量，抗氧化活性和抗菌活性之间的关系，这表明酚类化合物的存在可能解释的生物效应。HPLC分析显示B的水提取物中含有高水平的原儿茶酸、尿黑酸、连苯三酚、没食子酸、对儿茶素和二羟基苯甲酸。edulis，解释了最高的抗微生物和抗微生物膜性能。重要的是，蘑菇提取物在所有测试浓度下均无细胞毒性。总的来说，测试的蘑菇提取物是进一步探索其在预防伤口感染，特别是多重耐药病原体中的用途的良好候选物。
Multidrug-resistant ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species) has become the most recurrent global cause of skin and soft-tissue infections, belonging to the WHO priority pathogens list. Successful therapy remains challenging and entails the assessment of novel and successful antibiotics. In this study, mushrooms are considered a valuable and unique source of natural antimicrobial compounds. Therefore, this study aimed to evaluate the antimicrobial and antibiofilm properties of Boletus edulis (B. edulis) and Neoboletus luridiformis (N. luridiformis) aqueous and methanolic extracts against ESKAPE isolates from clinical wound infections. Disk diffusion and microdilution methods were used to assess the antimicrobial activity. Phytochemical characterization was achieved by analysis of total phenols, orthodiphenols content, and antioxidant activity as well as by high-performance liquid chromatography-diode array detector (HPLC-DAD). Human foreskin fibroblasts-1 (HFF-1) cell viability was performed by the MTT assay. Aqueous and methanolic extracts of B. edulis and N. luridiformis showed antimicrobial and antibiofilm properties against multidrug-resistant bacteria, although with different efficacy rates. The results showed that there is a convincing relation between the content of phenolic compounds, antioxidant activity, and antimicrobial activity suggesting that the presence of phenolic compounds may explain the biological effects. HPLC analysis revealed high levels of protocatechuic acid, homogentisic acid, pyrogallol, gallic acid, p-catechin, and dihydroxybenzoic acid in the aqueous extract of B. edulis, explaining the highest antimicrobial and antibiofilm properties. Importantly, the mushrooms extracts were non-cytotoxic at all the tested concentrations. Overall, the tested mushrooms extracts are good candidates to further explore its use in the prevention of wound infection, particularly by multidrug-resistant pathogens.
登录
查看更多内容
DOI：
10.1155/2015/795435
发表时间：
2015
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Lopez-Romero JC;González-Ríos H;Borges A;Simões M
通讯作者：
Simões M
影响因子：
2.4
作者：
Gupta PD;Birdi TJ
通讯作者：
Birdi TJ
影响因子：
5.2
作者：
Bouarab-Chibane, Lynda;Forquet, Valerian;Bordes, Claire
通讯作者：
Bordes, Claire
影响因子：
1.1
作者：
Mathur P;Singh S
通讯作者：
Singh S
影响因子：
8.8
作者：
Yahia, Elhadi M.;Gutierrez-Orozco, Fabiola;Moreno-Perez, Marco A.
通讯作者：
Moreno-Perez, Marco A.