Antibacterial Effects and Mode of Action of Selected Essential Oils Components against Escherichia coli and Staphylococcus aureus.
Antibacterial Effects and Mode of Action of Selected Essential Oils Components against Escherichia coli and Staphylococcus aureus.
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DOI：
10.1155/2015/795435
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Simões M
中科院分区：
文献类型：
--
作者：
Lopez-Romero JC;González-Ríos H;Borges A;Simões M
细菌耐药性在世界范围内的报道越来越多，是感染性疾病治疗失败的主要原因之一。天然产品，包括植物次生代谢物（植物化学物质），可以用来克服或减少这个问题。本研究的目的是确定所选精油 (EO) 成分：香芹醇、香芹酮、香茅醇和香茅醛对大肠杆菌和金黄色葡萄球菌的抗菌作用和作用方式。对所选 EO 成分的最低抑菌浓度 (MIC) 和最低杀菌浓度 (MBC) 进行了评估。此外，还进行了细菌表面理化表征、细菌表面电荷、膜完整性和 K + 渗漏分析，以研究 EO 成分的抗菌作用模式。香茅醇是对抗这两种病原体最有效的分子，其次是香茅醛、香芹醇和香芹酮。暴露于 EO 后，观察到疏水性、表面电荷和膜完整性的变化以及随后大肠杆菌和金黄色葡萄球菌的 K + 泄漏。这项研究表明，所选的环氧乙烷对测试的细菌具有显着的抗菌活性，作用于细胞表面并导致细菌膜破裂。此外，这些分子是控制微生物感染的传统抗菌剂的有趣替代品。
Bacterial resistance has been increasingly reported worldwide and is one of the major causes of failure in the treatment of infectious diseases. Natural-based products, including plant secondary metabolites (phytochemicals), may be used to surpass or reduce this problem. The objective of this study was to determine the antibacterial effect and mode of action of selected essential oils (EOs) components: carveol, carvone, citronellol, and citronellal, against Escherichia coli and Staphylococcus aureus. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were assessed for the selected EOs components. Moreover, physicochemical bacterial surface characterization, bacterial surface charge, membrane integrity, and K + leakage assays were carried out to investigate the antimicrobial mode of action of EOs components. Citronellol was the most effective molecule against both pathogens, followed by citronellal, carveol, and carvone. Changes in the hydrophobicity, surface charge, and membrane integrity with the subsequent K + leakage from E. coli and S. aureus were observed after exposure to EOs. This study demonstrates that the selected EOs have significant antimicrobial activity against the bacteria tested, acting on the cell surface and causing the disruption of the bacterial membrane. Moreover, these molecules are interesting alternatives to conventional antimicrobials for the control of microbial infections.
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