Comparative Metabolic Characterization of Extraintestinal Pathogenic Escherichia coli Blood Isolates from Saudi Arabia.
Comparative Metabolic Characterization of Extraintestinal Pathogenic Escherichia coli Blood Isolates from Saudi Arabia.
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DOI：
10.1155/2022/1745835
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发表时间：
2022
影响因子：
2.2
通讯作者：
Alyousef, Abdullah A. A.
中科院分区：
文献类型：
--
作者：
Alangari, Abdulaziz;Jaffal, Ahmad Abu;Alyousef, Abdulaziz M. M.;Alyousef, Abdullah A. A.
由肠外致病性大肠埃希菌(ExPEC)引起的血液感染的患病率大幅上升。E.coliST131是大肠杆菌菌血症群体中最主要的ExPEC克隆之一。代谢可触发某些细菌分离株的致病作用，本文对β-内酰胺酶/超广谱β-内酰胺酶阳性和ESBL-阴性菌株、ST131和非ST131菌株的代谢特性进行了评价和比较。用HiMedia碳水化合物试纸测定了利雅得一家三级医疗机构住院患者的血液样本中分离的30株大肠杆菌的代谢特征。然后对隔离组之间的利用能力差异进行统计评估。我们的数据发现，与ESBL阳性菌株相比，非BL/ESBL产生菌的代谢能力较低，尽管差异仍然不显着。与非ST131菌株相比，ST131菌株对果糖和海藻糖等碳水化合物的利用水平更高，而且ST131菌株对鼠李糖的代谢也显著相关。两个分离物组的平均生物得分均不显著。我们发现在被测试的血液分离株中，新陈代谢和抗菌药敏感性之间没有联系。ST131血分离株的碳水化合物利用活性略高于非ST131血分离株。更重要的是，ST131菌株能够显著代谢鼠李糖。未来的研究应该集中在可能推动ST131等主要ExPEC克隆成功的因素上。
The prevalence of bloodstream infections caused by extraintestinal pathogenic Escherichia coli (ExPEC) has increased substantially. E. coli ST131 is one of the dominant ExPEC clones among E. coli bacteremia population. Metabolism can trigger the pathogenesis of some bacterial isolates, and here we evaluated and compared the metabolic traits of E. coli bacteremia isolates including β-lactamase (BL)/extended-spectrum β-lactamase (ESBL)-positive and ESBL-negative isolates and ST131 and non-ST131 isolates. The metabolic profiles of thirty E. coli isolates, obtained from blood samples for hospitalized individuals at a tertiary healthcare facility in Riyadh, were determined using HiMedia carbohydrate test strips. The difference in the utilization ability between isolate groups was then statistically assessed. Our data found that non-BL/ESBL producers were of low metabolic capacity compared with ESBL-positive isolates although the difference remained insignificant. Higher levels of utilization for some carbohydrates, such as fructose and trehalose, were detected among ST131 isolates when compared with non-ST131, and ST131 was also significantly associated with metabolizing rhamnose. The mean bio-score of both isolate groups was insignificant. We showed no link between metabolism and antimicrobial susceptibility profiles among tested blood isolates. ST131 blood isolates were slightly higher in their carbohydrate utilization activity than non-ST131. More importantly, ST131 isolates were significantly capable of metabolizing rhamnose. Future research should focus on the factors that might drive the success of major ExPEC clones such as ST131.
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影响因子：
5.7
作者：
Al-Agamy MH;Shibl AM;Hafez MM;Al-Ahdal MN;Memish ZA;Khubnani H
通讯作者：
Khubnani H
影响因子：
4.2
作者：
Johnson, James R.;Porter, Stephen;Castanheira, Mariana
通讯作者：
Castanheira, Mariana
影响因子：
5.2
作者：
Alhashash, Fahad;Wang, Xiaohui;Mcnally, Alan
通讯作者：
Mcnally, Alan
影响因子：
5.2
作者：
Croxall, Gemma;Hale, James;McNally, Alan
通讯作者：
McNally, Alan
影响因子：
9.4
作者：
Gibreel, Tarek M.;Dodgson, Andrew R.;Upton, Mathew
通讯作者：
Upton, Mathew