The relationship between fasting-induced torpor, sleep and wakefulness in the laboratory mouse
The relationship between fasting-induced torpor, sleep and wakefulness in the laboratory mouse
复制标题
实验小鼠禁食引起的麻木、睡眠和觉醒之间的关系
DOI：
10.1101/2020.05.05.076570
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Huang Y
中科院分区：
文献类型：
--
作者：
Huang Y
研究目的Torpor是一种调节和可逆的代谢抑制状态，被许多哺乳动物用来保存能量。虽然在季节性冬眠者中，昏睡与睡眠之间的关系已经得到了很好的研究，但在实验室小鼠中，禁食引起的麻木对警觉状态和脑活动的影响却知之甚少。方法连续几天对成年、雄性C57BL/6小鼠进行脑电(EEG)、肌电(EMG)和体表温度的监测。尽管非快速眼动(NREM)睡眠、快速眼动(REM)睡眠和清醒状态的典型电生理特征使我们能够在所有情况下进行警觉状态分类，但EEG和EMG受到低温的显著影响。与之前的研究一致，低体温发作是在一种与NREM睡眠难以区分的状态下开始的，脑电功率随着体表温度的下降而逐渐下降。在深度低温期间，REM睡眠基本上被取消，我们观察到与颤抖相关的肌肉活动的强烈爆发。结论我们的研究强调了禁食引起的小鼠麻木、丛林仓鼠的日常麻木和季节性冬眠物种的冬眠的脑电特征之间的重要相似性。需要进一步的研究来阐明禁食引起的麻木对随后睡眠的影响。
Study ObjectivesTorpor is a regulated and reversible state of metabolic suppression used by many mammalian species to conserve energy. Whereas the relationship between torpor and sleep has been well-studied in seasonal hibernators, less is known about the effects of fasting-induced torpor on states of vigilance and brain activity in laboratory mice.MethodsContinuous monitoring of electroencephalogram (EEG), electromyogram (EMG), and surface body temperature was undertaken in adult, male C57BL/6 mice over consecutive days of scheduled restricted feeding.ResultsAll animals showed bouts of hypothermia that became progressively deeper and longer as fasting progressed. EEG and EMG were markedly affected by hypothermia, although the typical electrophysiological signatures of non-rapid eye movement (NREM) sleep, rapid eye movement (REM) sleep, and wakefulness enabled us to perform vigilance-state classification in all cases. Consistent with previous studies, hypothermic bouts were initiated from a state indistinguishable from NREM sleep, with EEG power decreasing gradually in parallel with decreasing surface body temperature. During deep hypothermia, REM sleep was largely abolished, and we observed shivering-associated intense bursts of muscle activity.ConclusionsOur study highlights important similarities between EEG signatures of fasting-induced torpor in mice, daily torpor in Djungarian hamsters and hibernation in seasonally hibernating species. Future studies are necessary to clarify the effects on fasting-induced torpor on subsequent sleep.
登录
查看更多内容
冷防御神经通路驱动压力诱发的高热
DOI：
--
发表时间：
2015
期刊：
Autonomic Neuroscience
影响因子：
--
作者：
K. Nakamura
通讯作者：
K. Nakamura
影响因子：
5.6
作者：
Kolli R;Soll J;Carrie C
通讯作者：
Carrie C
影响因子：
2.6
作者：
BERGER, RJ
通讯作者：
BERGER, RJ
睡眠剥夺和麻木对正加仓鼠脑电图慢波特征的不同影响
影响因子：
3.7
作者：
V. Vyazovskiy;S. Palchykova;Peter Achermann;I. Tobler;T. Deboer
通讯作者：
T. Deboer
影响因子：
5.3
作者：
von der Ohe, Christina G.;Darian-Smith, Corinna;Heller, H. Craig
通讯作者：
Heller, H. Craig