Bestrophin detected in the basal membrane of the retinal epithelium and drusen of monkeys with drusenoid maculopathy.
Bestrophin detected in the basal membrane of the retinal epithelium and drusen of monkeys with drusenoid maculopathy.
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DOI：
10.1007/s00417-009-1091-9
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发表时间：
2009-08
期刊：
影响因子：
--
通讯作者：
Mattison JA
中科院分区：
文献类型：
--
作者：
Gouras P;Braun K;Ivert L;Neuringer M;Mattison JA
目的：确定黄斑视网膜色素上皮（RPE）基底膜和年龄相关性黄斑样病变恒河猴的drusenoid。用4%多聚甲醛固定眼，对3只23 ~ 24岁的黄斑样黄斑病恒河猴（猕猴）的黄斑区进行解剖。黄斑被切成长方形。每个节段去除巩膜，其余部分分成带视网膜上皮的神经视网膜节段或带视网膜上皮的脉络膜节段。这些片段与山羊抗人抗strophin 1多克隆抗体孵育，与金标记的兔抗山羊IgG反应，银增强，并进行透射电镜处理。在黄斑RPE基底表面发现了标记金颗粒的准线性排列，在膜样物质片段中也发现了标记金颗粒。在上皮基膜上，与金颗粒相连的besstrophin的排列密度最大值约为100个besstrophin分子/微米2。当在附着于神经视网膜的RPE层中进行检测时，免疫检测最有效，其中上皮的基底表面更直接暴露于抗体。在年龄相关性黄斑样病变的恒河猴黄斑RPE的基底膜上也发现了Bestrophin，并且在drusen的膜样结构中也发现了该蛋白。后一项发现通过表明RPE基膜片段有助于drusen内积累的物质，为drusen的发病机制提供了深入的了解。
To determine if bestrophin is present in the basal membrane of macular retinal pigment epithelium (RPE) and in drusen of rhesus monkeys with age related drusenoid maculopathy. The macular region of three rhesus monkeys (Macaca mulatta), 23–24 years of age, with drusenoid maculopathy was dissected from eyes fixed with 4% paraformaldehyde. The macula was sectioned into rectangular pieces. The sclera was removed from each segment and the remainder separated into segments of neural retina with retinal epithelium or choroid with retinal epithelium. These segments were incubated with a goat polyclonal antibody to human bestrophin 1, reacted with gold-labeled rabbit antibody to goat IgG, silver enhanced, and processed for transmission electron microscopy. Bestrophin labeled gold particles were found in quasi-linear arrays on the basal surface of the macular RPE and also within drusen where bestrophin was found in segments of membranous-like material. The array density of the bestrophin linked gold particles on the basal membrane of the epithelium had a maximal value of about 100 bestrophin molecules/micron2. Immuno-detection of bestrophin was most effective when examined in an RPE layer that remained attached to the neural retina, where the basal surface of the epithelium is more directly exposed to the antibodies. Bestrophin is present on the basal membrane of macular RPE of rhesus monkeys with age related drusenoid maculopathy and also found in the membranous-like structures of drusen. The latter finding provides insight into the pathogenesis of drusen by indicating that segments of the basal membrane of RPE contribute to the material that accumulates within drusen.
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DOI：
10.1007/s00417-008-0910-8
发表时间：
2008-10
期刊：
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie
影响因子：
--
作者：
Gouras P;Ivert L;Landauer N;Mattison JA;Ingram DK;Neuringer M
通讯作者：
Neuringer M
影响因子：
4.4
作者：
Mullins, Robert F.;Kuehn, Markus H.;Stone, Edwin M.
通讯作者：
Stone, Edwin M.
影响因子：
30.8
作者：
Petrukhin, K;Koisti, MJ;Wadelius, C
通讯作者：
Wadelius, C
DOI：
10.1007/s00417-008-0911-7
发表时间：
2008-10
期刊：
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie
影响因子：
--
作者：
Gouras P;Ivert L;Mattison JA;Ingram DK;Neuringer M
通讯作者：
Neuringer M
影响因子：
3.5
作者：
Marquardt, A;Stöhr, H;Weber, BHF
通讯作者：
Weber, BHF