A Moderately Thermophilic Mixed Microbial Culture for Bioleaching of Chalcopyrite Concentrate at High Pulp Density
A Moderately Thermophilic Mixed Microbial Culture for Bioleaching of Chalcopyrite Concentrate at High Pulp Density
复制标题
用于高矿浆密度下黄铜矿精矿生物浸出的中等高温混合微生物培养物
DOI：
10.1128/aem.02907-13
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发表时间：
2013-11
影响因子：
4.4
通讯作者：
Zhou Hongbo
中科院分区：
文献类型：
--
作者：
Wang Yuguang;Zeng Weimin;Qiu Guanzhou;Chen Xinhua;Zhou Hongbo
摘要：采集了中国不同地点的三种样品（酸性矿井排水、煤矿废水和温泉）。此后，将这些样品合并，然后接种到基础盐溶液中，其中不同的底物（硫酸亚铁、元素硫和黄铜矿）作为能源。之后，将在不同基质上生长的混合培养物平均汇集，形成最终的混合培养物。经过2年多的连续传代培养逐渐提高黄铜矿精矿的矿浆密度后，最终培养物能够在矿浆密度为20%（重量/体积）时有效浸出黄铜矿。在该矿浆密度下，培养物在 25 天内从黄铜矿中提取了 60.4% 的铜。通过变性梯度凝胶电泳和构建16S rRNA基因克隆文库分析适应过程中细菌和古菌的多样性。结果表明，在适应矿浆密度为4%之前，培养物主要由嗜铁钩端螺旋菌、嗜酸硫杆菌、嗜酸硫杆菌和嗜热铁原体4个菌种组成。然而，当纸浆密度大于4%时，无法检测到L. ferriphilum。采用实时定量 PCR 监测矿浆密度为 20% 的生物浸出过程中的微生物动态。结果表明，初期优势种为A. caldus，中期优势种为S.acidophilus，而非A. caldus。最后阶段嗜热镰刀菌所占比例最大。
ABSTRACT Three kinds of samples (acid mine drainage, coal mine wastewater, and thermal spring) derived from different sites were collected in China. Thereafter, these samples were combined and then inoculated into a basal salts solution in which different substrates (ferrous sulfate, elemental sulfur, and chalcopyrite) served as energy sources. After that, the mixed cultures growing on different substrates were pooled equally, resulting in a final mixed culture. After being adapted to gradually increasing pulp densities of chalcopyrite concentrate by serial subculturing for more than 2 years, the final culture was able to efficiently leach the chalcopyrite at a pulp density of 20% (wt/vol). At that pulp density, the culture extracted 60.4% of copper from the chalcopyrite in 25 days. The bacterial and archaeal diversities during adaptation were analyzed by denaturing gradient gel electrophoresis and constructing clone libraries of the 16S rRNA gene. The results show that the culture consisted mainly of four species, including Leptospirillum ferriphilum, Acidithiobacillus caldus, Sulfobacillus acidophilus, and Ferroplasma thermophilum, before adapting to a pulp density of 4%. However, L. ferriphilum could not be detected when the pulp density was greater than 4%. Real-time quantitative PCR was employed to monitor the microbial dynamics during bioleaching at a pulp density of 20%. The results show that A. caldus was the predominant species in the initial stage, while S. acidophilus rather than A. caldus became the predominant species in the middle stage. F. thermophilum accounted for the greatest proportion in the final stage.
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影响因子：
4.7
作者：
Watling, H. R.;Perrot, F. A.;Shiers, D. W.
通讯作者：
Shiers, D. W.
DOI：
--
发表时间：
2003-03
期刊：
Journal of The South African Institute of Mining and Metallurgy
影响因子：
--
作者：
A. Sissing;S. Harrison
通讯作者：
A. Sissing;S. Harrison
DOI：
10.1007/bf02654471
发表时间：
1981-01-01
期刊：
METALLURGICAL TRANSACTIONS B-PROCESS METALLURGY
影响因子：
--
作者：
DUTRIZAC, JE
通讯作者：
DUTRIZAC, JE
影响因子：
4.1
作者：
Q. Zhou;Fu Bo;Zhou Hong Bo;L. Xi;G. Jian;Liu Fei Fei-Liu-Fei-Fei-138085066;Chen Xin Hua
通讯作者：
Q. Zhou;Fu Bo;Zhou Hong Bo;L. Xi;G. Jian;Liu Fei Fei-Liu-Fei-Fei-138085066;Chen Xin Hua
影响因子：
1.4
作者：
M. Wei;Ru-bing Zhang;Yuguang Wang;Hou Ji;Jia Zheng;Xinhua Chen;Hong-bo Zhou
通讯作者：
M. Wei;Ru-bing Zhang;Yuguang Wang;Hou Ji;Jia Zheng;Xinhua Chen;Hong-bo Zhou