A role of IgM antibodies in monosodium urate crystal formation and associated adjuvanticity.
A role of IgM antibodies in monosodium urate crystal formation and associated adjuvanticity.
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IgM抗体在单钠晶体晶体形成和相关辅助性中的作用。
DOI：
10.4049/jimmunol.0803777
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发表时间：
2009-02-15
期刊：
影响因子：
--
通讯作者：
Shi Y
中科院分区：
文献类型：
--
作者：
Kanevets U;Sharma K;Dresser K;Shi Y
尿酸从受损细胞中释放出来，可以作为免疫系统的辅助信号。尿酸结晶在组织中引起强烈的炎症反应。虽然它们的生物学效应很明显，相关的信号机制也越来越清楚，但尿酸在体内快速沉淀的原因仍未得到解释，而在体外则形成了最小结晶。我们在这里报道了一组IgM抗体能够与这些晶体结合，这是有趣的，因为B细胞缺陷小鼠不能感知尿酸的促炎辅助作用。这些抗体的滴度在尿酸结晶免疫后增加。我们已经生产了大量的这种单克隆抗体。纯化后的IgM抗体能显著促进体外尿酸沉淀形成炎性结晶体。将这些抗体输注到B细胞缺陷小鼠体内，可显著增加这些受体的基础炎症水平，并恢复宿主感知尿酸佐剂性的能力。因此，我们已经确定了一个决定尿酸沉淀的因素，并可能确定其作为内源性佐剂的能力。这一发现提示了痛风性关节炎发病机制和尿酸诱导免疫激活的新机制。
Uric acid is released from injured cells and can act as an adjuvant signal to the immune system. Uric acid crystals invoke strong inflammatory responses in tissues. While their biological effects are evident and the associated signalling mechanisms are becoming clear, it remains unexplained as to why uric acid precipitates rapidly in vivo, in sharp contrast to the minimal crystallization in vitro. We report here that a group of IgM antibodies are able to bind to these crystals, which is interesting in light that B cell deficient mice do not sense the proinflammatory adjuvant effect of uric acid. The titers of these antibodies increase upon immunization with uric acid crystals. We have produced large quantities of such monoclonal antibodies. The purified IgM antibodies can significantly facilitate uric acid precipitation to form the inflammatory crystals in vitro. Infusion of these antibodies into B cell deficient mice significantly increases the basal level of inflammation in these recipients and restores the host’s ability to sense uric acid’s adjuvanticity. Therefore, we have identified a factor in determining uric acid precipitation and possibly its ability to function as an endogenous adjuvant. This finding suggests a new mechanism of the pathogenesis of gouty arthritis and uric acid induced immune activation.
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影响因子：
6.6
作者：
IWATA, H;NISHIO, S;TAKEUCHI, M
通讯作者：
TAKEUCHI, M
DOI：
10.1073/pnas.78.11.6858
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
AMES, BN;CATHCART, R;HOCHSTEIN, P
通讯作者：
HOCHSTEIN, P
影响因子：
--
作者：
LOEB, JN
通讯作者：
LOEB, JN
影响因子：
56.9
作者：
Dostert, Catherine;Petrilli, Virginie;Tschopp, Jurg
通讯作者：
Tschopp, Jurg
影响因子：
4.8
作者：
KAM, M;PERLTREVES, D;ADDADI, L
通讯作者：
ADDADI, L