Genome sequence of the necrotrophic plant pathogen Pythium ultimum reveals original pathogenicity mechanisms and effector repertoire.
Genome sequence of the necrotrophic plant pathogen Pythium ultimum reveals original pathogenicity mechanisms and effector repertoire.
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DOI：
10.1186/gb-2010-11-7-r73
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发表时间：
2010
期刊：
影响因子：
12.3
通讯作者：
Buell CR
中科院分区：
文献类型：
--
作者：
Lévesque CA;Brouwer H;Cano L;Hamilton JP;Holt C;Huitema E;Raffaele S;Robideau GP;Thines M;Win J;Zerillo MM;Beakes GW;Boore JL;Busam D;Dumas B;Ferriera S;Fuerstenberg SI;Gachon CM;Gaulin E;Govers F;Grenville-Briggs L;Horner N;Hostetler J;Jiang RH;Johnson J;Krajaejun T;Lin H;Meijer HJ;Moore B;Morris P;Phuntmart V;Puiu D;Shetty J;Stajich JE;Tripathy S;Wawra S;van West P;Whitty BR;Coutinho PM;Henrissat B;Martin F;Thomas PD;Tyler BM;De Vries RP;Kamoun S;Yandell M;Tisserat N;Buell CR
终极腐霉（Pythium ultimum）是一种普遍存在的卵菌植物病原菌，可引起多种作物和观赏植物的病害。终极疫霉基因组（42.8 Mb）编码15，290个基因，并且与相关的疫霉属物种（包括马铃薯枯萎病病原体致病疫霉（Phytophthora infestans））具有广泛的序列相似性和同线性。全转录组测序显示86%的基因表达，在非生物胁迫和宿主存在下，可检测到基因组的差异表达。预测的蛋白质组包括大量参与植物病原体相互作用的蛋白质，尽管令人惊讶的是，与相关的植物病原卵菌相比，终极拟青霉基因组不编码任何经典的RXLR效应子和相对较少的Crinkler基因。与疫霉属物种相比，参与碳水化合物代谢的酶的数量较少，角质酶明显缺乏，表明终极疫霉和更具宿主特异性的卵菌物种之间的毒力机制存在显著差异。虽然我们观察到与疫霉基因组的高度同源性，但终极疫霉蛋白质组具有新的特征，包括参与蛋白质水解的基因和腐霉特有基因的扩增。我们确定了一个小的基因家族的钙粘蛋白，蛋白质参与细胞粘附，这些基因组外的后生动物的第一份报告。对终极疫霉基因组的访问不仅揭示了卵菌纲内的核心致病机制，而且还揭示了与霜霉科相比，腐皮科谱系内发现的替代毒力和生活方式相关的谱系特异性基因。
Pythium ultimum is a ubiquitous oomycete plant pathogen responsible for a variety of diseases on a broad range of crop and ornamental species. The P. ultimum genome (42.8 Mb) encodes 15,290 genes and has extensive sequence similarity and synteny with related Phytophthora species, including the potato blight pathogen Phytophthora infestans. Whole transcriptome sequencing revealed expression of 86% of genes, with detectable differential expression of suites of genes under abiotic stress and in the presence of a host. The predicted proteome includes a large repertoire of proteins involved in plant pathogen interactions, although, surprisingly, the P. ultimum genome does not encode any classical RXLR effectors and relatively few Crinkler genes in comparison to related phytopathogenic oomycetes. A lower number of enzymes involved in carbohydrate metabolism were present compared to Phytophthora species, with the notable absence of cutinases, suggesting a significant difference in virulence mechanisms between P. ultimum and more host-specific oomycete species. Although we observed a high degree of orthology with Phytophthora genomes, there were novel features of the P. ultimum proteome, including an expansion of genes involved in proteolysis and genes unique to Pythium. We identified a small gene family of cadherins, proteins involved in cell adhesion, the first report of these in a genome outside the metazoans. Access to the P. ultimum genome has revealed not only core pathogenic mechanisms within the oomycetes but also lineage-specific genes associated with the alternative virulence and lifestyles found within the pythiaceous lineages compared to the Peronosporaceae.
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影响因子：
56.9
作者：
Armbrust, EV;Berges, JA;Rokhsar, DS
通讯作者：
Rokhsar, DS
影响因子：
6.5
作者：
Buckeridge, MS;dos Santos, HP;Tiné, MAS
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Tiné, MAS
影响因子：
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作者：
Brunner, F;Rosahl, S;Nürnberger, T
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Nürnberger, T
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作者：
Bairoch, Amos;Bougueleret, Lydie;Zhang, Jian
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影响因子：
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作者：
Boudjeko, T;Andème-Onzighi, C;Driouich, A
通讯作者：
Driouich, A