N-Acylhomoserine lactone-mediated quorum sensing regulates biofilm structure in Methylobacterium populi P-1M, an isolate from a pink-pigmented household biofilm
N-Acylhomoserine lactone-mediated quorum sensing regulates biofilm structure in Methylobacterium populi P-1M, an isolate from a pink-pigmented household biofilm
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N-酰基高丝氨酸内酯介导的群体感应调节人民甲基杆菌 P-1M（粉红色家用生物膜的分离物）中的生物膜结构
DOI：
10.1080/09168451.2018.1518701
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Ikeda Tsukasa
中科院分区：
文献类型：
--
作者：
Morohoshi Tomohiro;Xie Xiaonan;Ikeda Tsukasa
许多革兰氏阴性细菌都有群体感应系统，并产生AHL作为群体感应信号分子。在这项研究中，我们证明了从粉红色家居生物膜中分离到的一株甲氧半乳杆菌P-1M产生了两种AHL，C14：1-HSL为主要产物，3uch14-HSL为次要产物。Fm的完整基因组序列。PopiP-1M揭示了编码AHL合成酶(MpoI)和AHL受体(MpoR)的基因的存在。与之相反，缺失突变体形成的生物膜表面呈波浪状结构，与玻璃管的粘附性较强。在突变株中加入C14：1-HSL，其生物膜结构与野生型相似。这些结果证明了Fm的结构和粘接强度。缩写：AHL：N-酰基-L-高丝氨酸内酯；C14：1-HSL：N-十四烯酰基-L-高丝氨酸内酯；3OHC14-HSL:N-(3-hydroxytetradecanoyl)-l-homoserine内酯；SAM：S-腺苷-L-蛋氨酸；ACP：酰基载体蛋白；EPS：胞外多糖；DMSO：二甲基亚砜
Numerous gram-negative bacteria have quorum-sensing systems and produce AHL as a quorum-sensing signal molecule. In this study, we demonstrated thatMethylobacterium populiP-1M, an isolate from a pink-pigmented household biofilm, produced two AHLs, C14:1-HSL as a predominant product and 3OHC14-HSL as a minor product. The complete genome sequence ofM. populiP-1M revealed the presence of genes that are predicted to encode an AHL synthase (mpoI) and AHL receptor (mpoR).M. populiP-1M formed a pellicle-like biofilm, which had a flat surface and was easily removable. In contrast, biofilms formed bympoIand/ormpoRdeletion mutants had a wavy surface structure and strongly adhered to the glass tube. When C14:1-HSL was added to thempoImutant culture, the biofilm structure resembled that of the wild-type strain. These results demonstrated that the structure and adhesion strength ofM. populiP-1M biofilms are determined in part by AHL-mediated quorum sensing.Abbreviations:AHL:N-acyl-l-homoserine lactone; C14:1-HSL:N-tetradecenoyl-l-homoserine lactone; 3OHC14-HSL:N-(3-hydroxytetradecanoyl)-l-homoserine lactone; SAM:S-adenosyl-l-methionine; ACP: acyl-acyl carrier protein; EPS: extracellular polysaccharide; DMSO: dimethyl sulfoxide
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DOI：
10.1073/pnas.97.16.8789
发表时间：
2000-08-01
影响因子：
11.1
作者：
Parsek, MR;Greenberg, EP
通讯作者：
Greenberg, EP
影响因子：
5.1
作者：
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通讯作者：
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影响因子：
2.6
作者：
K. Furuhata;K. Goto;Y. Kato;Keiko Saitou;J. Sugiyama;M. Hara;S. Yoshida;M. Fukuyama
通讯作者：
M. Fukuyama
影响因子：
4.4
作者：
Tomlin, KL;Malott, RJ;Ceri, H
通讯作者：
Ceri, H
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影响因子：
3.2
作者：
C. G. N. Penalver;F. Cantet;D. Morin;D. Haras;J. Vorholt
通讯作者：
J. Vorholt