miR-148a promoted cell proliferation by targeting p27 in gastric cancer cells.
miR-148a promoted cell proliferation by targeting p27 in gastric cancer cells.
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miR-148a 通过靶向胃癌细胞中的 p27 促进细胞增殖
DOI：
10.7150/ijbs.7.567
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发表时间：
2011-05-05
影响因子：
9.2
通讯作者：
Yang X
中科院分区：
文献类型：
--
作者：
Guo SL;Peng Z;Yang X;Fan KJ;Ye H;Li ZH;Wang Y;Xu XL;Li J;Wang YL;Teng Y;Yang X
越来越多的证据表明，miRNAs在人胃癌中表达异常，在胃癌的发生发展中起着重要作用。在此，我们确定了miR-148 a在胃细胞增殖中的作用。miR-148 a敲低抑制胃癌细胞系的细胞增殖。相反，miR-148 a过表达促进细胞增殖和细胞周期进程。p27是细胞周期的关键抑制因子，miR-148 a的作用靶点为p27，提示miR-148 a可能通过下调p27的表达来促进胃癌细胞的增殖。此外，我们证实了miR-148 a在人类进展期胃癌组织中的表达频繁且显著下调，并观察到肿瘤样本中miR-148 a和p27表达之间存在良好的负相关性。因此，我们的研究结果表明，miR-148 a下调可能发挥某种拮抗作用的细胞增殖，而不是促进细胞增殖在胃癌。
Accumulating evidence has shown that miRNAs are aberrantly expressed in human gastric cancer and crucial to tumorigenesis. Herein, we identified the role of miR-148a in gastric cell proliferation. miR-148a knockdown inhibited cell proliferation in gastric cancer cell lines. Conversely, miR-148a overexpression promoted cell proliferation and cell cycle progression. p27, a key inhibitor of cell cycle, was verified as the target of miR-148a, indicating miR-148a might downregulate p27 expression to promote gastric cell proliferation. Moreover, we confirmed that miR-148a expression was frequently and dramatically downregulated in human advanced gastric cancer tissues, and observed a good inverse correlation between miR-148a and p27 expression in tumor samples. Thus, our results demonstrated that miR-148a downregulation might exert some sort of antagonistic function in cell proliferation, rather than promote cell proliferation in gastric cancer.
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影响因子：
13.5
作者：
Braconi, Chiara;Huang, Nianyuan;Patel, Tushar
通讯作者：
Patel, Tushar
DOI：
10.1111/j.1440-1746.2008.05666.x
发表时间：
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作者：
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影响因子：
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作者：
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通讯作者：
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