Localization of AQP5 during development of the mouse submandibular salivary gland.
Localization of AQP5 during development of the mouse submandibular salivary gland.
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DOI：
10.1007/s10735-010-9308-0
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发表时间：
2011-02
影响因子：
3.2
通讯作者：
Galtung, Hilde Kanli
中科院分区：
文献类型：
--
作者：
Larsen, Helga S.;Aure, Marit H.;Peters, Sarah B.;Larsen, Melinda;Messelt, Edward B.;Galtung, Hilde Kanli
已知水通道蛋白5（AQP 5）是唾液分泌的中心。研究AQP 5在下颌下腺发育和成体组织中的时空分布可能为进一步揭示其在发育过程中的未知作用提供线索。在本工作中，SMG从胚胎（E）14.5-18.5和出生后（P）0，2，5，25，和60天的AQP 5免疫染色，并使用光学显微镜分析。对P60腺体进行额外的共聚焦和透射电子显微镜检查。我们的研究结果表明，AQP 5的表达首先发生在一个分散的模式在晚期小管阶段，并变得更加突出和组织在终端微管/前腺泡细胞对出生。出生前发现整个小叶内导管的顶膜染色。在出生后的发育过程中，AQP 5在前腺泡/腺泡细胞的腔膜和侧膜中表达。AQP 5在P25和P60的腺泡细胞的基底膜中也被检测到。在P60的闰管中，雄性腺体在整个节段中显示顶端染色，而雌性腺体仅近端区域呈阳性。这些结果表明，AQP 5在出生前和出生后的发展过程中，在小鼠SMG的不断变化的分布。
Aquaporin 5 (AQP5) is known to be central for salivary fluid secretion. A study of the temporal-spatial distribution of AQP5 during submandibular gland (SMG) development and in adult tissues might offer further clues to its unknown role during development. In the present work, SMGs from embryonic day (E) 14.5–18.5 and postnatal days (P) 0, 2, 5, 25, and 60 were immunostained for AQP5 and analyzed using light microscopy. Additional confocal and transmission electron microscopy were performed on P60 glands. Our results show that AQP5 expression first occurs in a scattered pattern in the late canalicular stage and becomes more prominent and organized in the terminal tubuli/pro-acinar cells towards birth. Additional apical membrane staining in the entire intralobular duct is found just prior to birth. During postnatal development, AQP5 is expressed in both the luminal and lateral membrane of pro-acinar/acinar cells. AQP5 is also detected in the basal membrane of acinar cells at P25 and P60. In the intercalated ducts at P60, the male glands show apical staining in the entire segment, while only the proximal region is positive in the female glands. These results demonstrate an evolving distribution of AQP5 during pre- and postnatal development in the mouse SMGs.
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影响因子：
2.7
作者：
Daley, William P.;Gulfo, Kathryn M.;Sequeira, Sharon J.;Larsen, Melinda
通讯作者：
Larsen, Melinda
DOI：
10.1152/ajpgi.00480.2003
发表时间：
2004-07-01
影响因子：
4.5
作者：
Gresz, V;Kwon, TH;Nielsen, S
通讯作者：
Nielsen, S
影响因子：
4.8
作者：
Ma, TH;Song, YL;Verkman, AS
通讯作者：
Verkman, AS
影响因子：
4.5
作者：
Akamatsu, T;Parvin, MN;Hosoi, K
通讯作者：
Hosoi, K
影响因子：
2.7
作者：
Larsen, M;Hoffman, MP;Yamada, KM
通讯作者：
Yamada, KM