Prevention of medical device infections via multi-action nitric oxide and chlorhexidine diacetate releasing medical grade silicone biointerfaces.
Prevention of medical device infections via multi-action nitric oxide and chlorhexidine diacetate releasing medical grade silicone biointerfaces.
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DOI：
10.1002/jbm.a.37372
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发表时间：
2022-06
期刊：
影响因子：
--
通讯作者：
Brisbois EJ
中科院分区：
文献类型：
--
作者：
Chug MK;Massoumi H;Wu Y;Brisbois EJ
医疗器械表面细菌和生物膜的存在与严重感染、医疗保健成本增加和医疗器械失效有关。因此，迫切需要能够阻止微生物附着和生物被膜形成的抗菌生物界面和医疗设备。一氧化氮(NO)和二乙酸氯己定(CHXD)都具有广谱抗菌活性。在过去，CHXD和NO供体S-亚硝基-N-乙酰青霉胺(SNAP)结合的聚合物平台的单独聚合物释放系统在生物医学/治疗应用中引起了极大的关注。然而，这两个表面的结合还没有被探索过。在此，评价了NO和CHXD的协同作用，以制备抗菌医用硅橡胶(SR)。用CHXD(1、3和5wt%)作为面漆，用溶剂浇铸法制备了10wt%的SNAP薄膜，以产生双活性抗菌界面。化学发光研究证实SNAP-CHXD膜在生理水平(0.5~4×10−10molmin−1 cm−2)至少3周内释放NO，CHXD膜释放至少7d，进一步的扫描电子显微镜-能谱分析证实SNAP在聚合物膜中分布均匀且存在CHXD，接触角滞后证实了这一点.此外，与对照组相比，双活性SNAP-CHXD薄膜能够显著减少大肠杆菌和金黄色葡萄球菌(>；3-log减少)，而对小鼠成纤维细胞没有明显的毒性。这两种强大的抗菌剂之间的协同作用将有助于对抗生物界面上的细菌污染，并提高医疗器械的寿命。S-亚硝基-N-乙酰青霉胺(SNAP)和二醋酸氯己定(CHXD)在减少细菌污染和提高医疗器械耐用性方面具有协同作用。这两种杀菌化合物的多重作用导致活菌在表面定植的~gt；3log减少。
The presence of bacteria and biofilm on medical device surfaces has been linked to serious infections, increased health care costs, and failure of medical devices. Therefore, antimicrobial biointerfaces and medical devices that can thwart microbial attachment and biofilm formation are urgently needed. Both nitric oxide (NO) and chlorhexidine diacetate (CHXD) are possess broad-spectrum antibacterial properties. In the past, individual polymer release systems of CHXD and NO donor S-nitroso-N-acetylpenicillamine (SNAP) incorporated polymer platforms have attracted considerable attention for biomedical/therapeutic applications. However, the combination of the two surfaces has not yet been explored. Herein, the synergy of NO and CHXD was evaluated to create an antimicrobial medical-grade silicone rubber (SR). The 10 wt% SNAP films were fabricated using solvent casting with a topcoat of CHXD (1, 3, and 5 wt%) to generate a dual-active antibacterial interface. Chemiluminescence studies confirmed the NO release from SNAP-CHXD films at physiologically relevant levels (0.5 – 4 × 10−10 mol min−1 cm−2) for at least 3 weeks and CHXD release for at least 7 d. Further characterization of the films via SEM-EDS confirmed uniform distribution of SNAP and presence of CHXD within the polymer films without substantial morphological changes, as confirmed by contact angle hysteresis. Moreover, the dual-active SNAP-CHXD films were able to significantly reduce E. coli and S. aureus bacteria (> 3-log reduction) compared to controls with no explicit toxicity towards mouse fibroblast cells. The synergy between the two potent antimicrobial agents will help combat bacterial contamination on biointerfaces and enhance the longevity of medical devices. Synergistic effect of S-nitroso-N-acetyl-penicillamine (SNAP) and Chlorhexidine diacetate (CHXD) to reduce bacterial contamination and enhance the durability of medical devices. The multi-action of the two bactericidal compounds leads to >3-log reduction in viable bacterial colonization on surface.
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影响因子：
9.7
作者：
Brisbois EJ;Major TC;Goudie MJ;Bartlett RH;Meyerhoff ME;Handa H
通讯作者：
Handa H
影响因子：
14
作者：
Hetrick, Evan M.;Shin, Jae Ho;Paul, Heather S.;Schoenfisch, Mark H.
通讯作者：
Schoenfisch, Mark H.
影响因子：
5.5
作者：
Baccolini V;Migliara G;Isonne C;Dorelli B;Barone LC;Giannini D;Marotta D;Marte M;Mazzalai E;Alessandri F;Pugliese F;Ceccarelli G;De Vito C;Marzuillo C;De Giusti M;Villari P
通讯作者：
Villari P
影响因子：
9.5
作者：
Grommersch BM;Pant J;Hopkins SP;Goudie MJ;Handa H
通讯作者：
Handa H
DOI：
10.1080/00914037.2016.1163570
发表时间：
2016
期刊：
International journal of polymeric materials
影响因子：
--
作者：
Goudie MJ;Brisbois EJ;Pant J;Thompson A;Potkay JA;Handa H
通讯作者：
Handa H