Biotemplated Synthesis and Characterization of Mesoporous Nitric Oxide-Releasing Diatomaceous Earth Silica Particles.
Biotemplated Synthesis and Characterization of Mesoporous Nitric Oxide-Releasing Diatomaceous Earth Silica Particles.
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DOI：
10.1021/acsami.7b15967
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发表时间：
2018-01-24
影响因子：
9.5
通讯作者：
Handa H
中科院分区：
文献类型：
--
作者：
Grommersch BM;Pant J;Hopkins SP;Goudie MJ;Handa H
硅藻土（DE）是一种纳米多孔二氧化硅材料，由结晶化的单细胞海藻组成，具有独特的机械、分子传输、光学和光子特性，在一系列生物医学应用中得到利用。DE在这些应用中的效用将通过并入一氧化氮（NO）技术来增强，该技术显示出调节基本的生理过程。在这项工作中，制备和表征的生物模板硅藻土为基础的一氧化氮输送支架首次被描述。评价了三种氨基硅烷[（3-氨基丙基）三乙氧基硅烷（APTES）、N-（6-氨基己基）氨基甲基三乙氧基硅烷（AHAMTES）和3-氨基丙基二甲基乙氧基硅烷（APDMES）]通过N-乙酰基-d-青霉胺（NAP）与硅藻土的共价连接使NO负载最大化的能力。使用APTES交联剂导致最大的NAP拴系到DE表面，并且NAP-DE通过亚硝化转化为释放NO的S-亚硝基-N-乙酰基-青霉胺（SNAP）-DE。化学发光法测得SNAP-DE的总NO载量为0.0372 ± 0.00791 μmol/mg。保留硅藻土的独特的介孔形态在整个衍生化证实了扫描电子显微镜。SNAP-DE对革兰氏阳性菌金黄色葡萄球菌的杀灭率为92.95%。基于WST-8的细胞毒性测试显示对小鼠成纤维细胞没有负面影响，证明了SNAP-DE的生物相容性潜力。释放NO的硅藻土的发展提供了一种独特的手段，将可调水平的NO递送到聚合物化学、组织工程、药物递送和伤口愈合领域的材料。
Diatomaceous earth (DE), a nanoporous silica material composed of fossilized unicellular marine algae, possesses unique mechanical, molecular transport, optical, and photonic properties exploited across an array of biomedical applications. The utility of DE in these applications stands to be enhanced through the incorporation of nitric oxide (NO) technology shown to modulate essential physiological processes. In this work, the preparation and characterization of a biotemplated diatomaceous earth-based nitric oxide delivery scaffold are described for the first time. Three aminosilanes [(3-aminopropyl)triethoxysilane (APTES), N-(6-aminohexyl)aminomethyltriethoxysilane (AHAMTES), and 3-aminopropyldimethylethoxysilane (APDMES)] were evaluated for their ability to maximize NO loading via the covalent attachment of N-acetyl-d-penicillamine (NAP) to diatomaceous earth. The use of APTES cross-linker resulted in maximal NAP tethering to the DE surface, and NAP-DE was converted to NO-releasing S-nitroso-N-acetyl-penicillamine (SNAP)-DE by nitrosation. The total NO loading of SNAP-DE was determined by chemiluminescence to be 0.0372 ± 0.00791 μmol/mg. Retention of diatomaceous earth’s unique mesoporous morphology throughout the derivatization was confirmed by scanning electron microscopy. SNAP-DE exhibited 92.95% killing efficiency against Gram-positive bacteria Staphylococcus aureus as compared to the control. The WST-8-based cytotoxicity testing showed no negative impact on mouse fibroblast cells, demonstrating the biocompatible potential of SNAP-DE. The development of NO releasing diatomaceous earth presents a unique means of delivering tunable levels of NO to materials across the fields of polymer chemistry, tissue engineering, drug delivery, and wound healing.
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影响因子：
9.7
作者：
Brisbois EJ;Major TC;Goudie MJ;Bartlett RH;Meyerhoff ME;Handa H
通讯作者：
Handa H
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通讯作者：
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通讯作者：
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