Isolation of point mutations in bacteriophage Mu attachment regions cloned in a lambda::mini-Mu phage.

Isolation of point mutations in bacteriophage Mu attachment regions cloned in a lambda::mini-Mu phage.
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分离 lambda::mini-Mu 噬菌体中克隆的噬菌体 Mu 附着区的点突变。

DOI:
10.1073/pnas.83.16.6012
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发表时间:
1986
影响因子:
11.1
通讯作者:
Howe,MM
Howe,MM
中科院分区:
综合性期刊1区
文献类型:
--
作者:
Burlingame,RP;Obukowicz,MG;Lynn,DL;Howe,MM

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21个衍生物的λ::迷你Mu噬菌体含有点突变的Mu附着区后,分离mutD诱变和选择从Mu特异性复制干扰λ生长的救济。DNA序列分析表明,一个单一的左端突变体遭受了T-C转换在位置1的Mu序列,而其余的20个右端突变体包含单一的碱基对插入或删除的末端19个碱基对。遗传分析表明,通过测序揭示的右端突变是减轻λ生长的复制抑制所必需的。这些突变体的性质表明,末端2-碱基对和亚末端8-碱基对反向重复序列是重要的μ特异性复制转座。
Twenty-one derivatives of a lambda::mini-Mu phage containing point mutations in the Mu attachment regions were isolated after mutD mutagenesis and selection for relief from Mu-specific replicative interference of lambda growth. DNA sequence analysis revealed that the single left-end mutant had suffered a T----C transition at position 1 of the Mu sequence, while the remaining 20 right-end mutants contained single base-pair insertions or deletions within the terminal 19 base pairs. A genetic assay showed that the right-end mutations revealed by sequencing were necessary for relief of the replicative inhibition of lambda growth. The properties of these mutants suggest that the terminal 2-base-pair and subterminal 8-base-pair inverted repeats are important for Mu-specific replicative transposition.
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发表时间: 1981-01-01
期刊: BIOCHIMICA ET BIOPHYSICA ACTA
影响因子: --
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